BRENDA - Enzyme Database

Mechanism of estrogen biosynthesis. Participation of multiple enzyme sites in placental aromatase hydroxylations

Fishman, J.; Goto, J.; J. Biol. Chem. 256, 4466-4471 (1981) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.14
19-hydroxyandrost-4-ene-3,17-dione
competitively inhibits the formation of 19-hydroxyandrost-4-ene-3,17-dione and estrogen
Homo sapiens
1.14.14.14
19-oxoandrost-4-ene-3,17-dione
competitively inhibits the formation of 19-oxoandrost-4-ene-3,17-dione and estrogen
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.14
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.14
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.14
19-hydroxyandrost-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
19-oxo-androst-4-ene-3,17-dione + 2 H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
19-oxoandrost-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
estrone + formate + H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins
-
725327
Homo sapiens
estrone + formate + 4 H2O + 3 oxidized flavoproteins
overall reaction
-
-
?
1.14.14.14
androst-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
19-hydroxyandrost-4-ene-3,17-dione + H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
additional information
the aromatization of androstenedione probably involves two successive hydroxylations at the C-19 methyl group, mediated by a common catalytic site with the third and rate-determining 2beta-hydroxylation taking place at a different enzyme site
725327
Homo sapiens
?
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.14
0.00038
-
19-hydroxyandrost-4-ene-3,17-dione
substrate 19-oxoandrost-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.00045
-
19-hydroxyandrost-4-ene-3,17-dione
substrate androst-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.0015
-
19-oxoandrost-4-ene-3,17-dione
substrate 19-hydroxyandrost-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.003
-
19-oxoandrost-4-ene-3,17-dione
substrate androst-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.14
19-hydroxyandrost-4-ene-3,17-dione
competitively inhibits the formation of 19-hydroxyandrost-4-ene-3,17-dione and estrogen
Homo sapiens
1.14.14.14
19-oxoandrost-4-ene-3,17-dione
competitively inhibits the formation of 19-oxoandrost-4-ene-3,17-dione and estrogen
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.14.14
0.00038
-
19-hydroxyandrost-4-ene-3,17-dione
substrate 19-oxoandrost-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.00045
-
19-hydroxyandrost-4-ene-3,17-dione
substrate androst-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.0015
-
19-oxoandrost-4-ene-3,17-dione
substrate 19-hydroxyandrost-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
1.14.14.14
0.003
-
19-oxoandrost-4-ene-3,17-dione
substrate androst-4-ene-3,17-dione, pH 7.0, 37°C
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.14
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.14
19-hydroxyandrost-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
19-oxo-androst-4-ene-3,17-dione + 2 H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
19-oxoandrost-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
estrone + formate + H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins
-
725327
Homo sapiens
estrone + formate + 4 H2O + 3 oxidized flavoproteins
overall reaction
-
-
?
1.14.14.14
androst-4-ene-3,17-dione + O2 + a reduced flavoprotein
-
725327
Homo sapiens
19-hydroxyandrost-4-ene-3,17-dione + H2O + an oxidized flavoprotein
-
-
-
?
1.14.14.14
additional information
the aromatization of androstenedione probably involves two successive hydroxylations at the C-19 methyl group, mediated by a common catalytic site with the third and rate-determining 2beta-hydroxylation taking place at a different enzyme site
725327
Homo sapiens
?
-
-
-
?