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Literature summary extracted from

  • Kung, J.; Seifert, J.; von Bergen, M.; Bolla, M.
    Cyclohexanecarboxyl-coenzyme a (CoA) and cyclohex-1-ene-1-carboxyl-CoA dehydrogenases, two enzymes involved in the fermentation of benzoate and crotonate in Syntrophus aciditrophicus (2013), J. Bacteriol., 195, 3193-3200.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.10 expressed in Escherichia coli BL 21 Star(DE3) OneShot cells Syntrophus aciditrophicus
1.3.8.11 expressed in Escherichia coli BL 21 Star(DE3) OneShot cells Syntrophus aciditrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.11 cyclohex-1-ene-1-carbonyl-CoA
-
 Syntrophus aciditrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.10 0.005
-
 cyclohex-1-ene-1-carbonyl-CoA Km less than 0.005 mM, at pH 7.0 and 30°C Syntrophus aciditrophicus
1.3.8.10 0.019
-
 cyclohex-1,5-diene-1-carbonyl-CoA at pH 7.0 and 30°C Syntrophus aciditrophicus
1.3.8.11 0.022
-
 cyclohexane-1-carbonyl-CoA at pH 7.0 and 30°C Syntrophus aciditrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.10 150000
-
 gel filtration Syntrophus aciditrophicus
1.3.8.11 42000
-
 4 * 42000, calculated from amino acid sequence Syntrophus aciditrophicus
1.3.8.11 150000
-
 recombinant enzyme, gel filtration Syntrophus aciditrophicus
1.3.8.11 155000
-
 native enzyme, gel filtration Syntrophus aciditrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate Syntrophus aciditrophicus
-
 cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate Syntrophus aciditrophicus SB
-
 cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.10 Syntrophus aciditrophicus
-
-
-
1.3.8.10 Syntrophus aciditrophicus SB
-
-
-
1.3.8.11 Syntrophus aciditrophicus
-
-
-
1.3.8.11 Syntrophus aciditrophicus SB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.10 Ni-chelating affinity column chromatography Syntrophus aciditrophicus
1.3.8.11 octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography Syntrophus aciditrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.10 2.1
-
 using (E)-2-cyclohex-1,5-diene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C Syntrophus aciditrophicus
1.3.8.10 9.5
-
 using cyclohex-1-ene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C Syntrophus aciditrophicus
1.3.8.11 2.1
-
 cell extract, at pH 7.0 and 30°C Syntrophus aciditrophicus
1.3.8.11 52
-
 after 25fold purification, at pH 7.0 and 30°C Syntrophus aciditrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.10 cyclohex-1,5-diene-1-carbonyl-CoA + Ti(III) citrate Ti(III) citrate (5mM) in the presence of methyl viologen (1 mM) serves as the electron donor Syntrophus aciditrophicus cyclohex-1-ene-1-carbonyl-CoA + oxidized Ti(III) citrate
-
 r
1.3.8.10 cyclohex-1,5-diene-1-carbonyl-CoA + Ti(III) citrate Ti(III) citrate (5mM) in the presence of methyl viologen (1 mM) serves as the electron donor Syntrophus aciditrophicus SB cyclohex-1-ene-1-carbonyl-CoA + oxidized Ti(III) citrate
-
 r
1.3.8.10 cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 r
1.3.8.10 cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus SB cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 r
1.3.8.10 cyclohexa-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus benzoyl-CoA + ferrocenium hexafluorophosphate
-
 ir
1.3.8.10 cyclohexa-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus SB benzoyl-CoA + ferrocenium hexafluorophosphate
-
 ir
1.3.8.10 additional information no activity with ChCoA, 6-hydroxycyclohex-1-en-1-carboxyl-CoA, butyryl-CoA, glutaryl-CoA, succinyl-CoA, or cyclohexane carboxylate Syntrophus aciditrophicus ?
-
 ?
1.3.8.10 additional information no activity with ChCoA, 6-hydroxycyclohex-1-en-1-carboxyl-CoA, butyryl-CoA, glutaryl-CoA, succinyl-CoA, or cyclohexane carboxylate Syntrophus aciditrophicus SB ?
-
 ?
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Syntrophus aciditrophicus SB cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.10 homotetramer 4 * 40000-45000, SDS-PAGE Syntrophus aciditrophicus
1.3.8.11 homotetramer 4 * 42000, calculated from amino acid sequence Syntrophus aciditrophicus

Synonyms

EC Number Synonyms Comment Organism
1.3.8.10 Ch1CoA dehydrogenase
-
 Syntrophus aciditrophicus
1.3.8.10 cyclohex-1-ene-1-carboxyl-CoA dehydrogenase
-
 Syntrophus aciditrophicus
1.3.8.10 cyclohexanecarboxyl-CoA dehydrogenase
-
 Syntrophus aciditrophicus
1.3.8.11 ChCoA dehydrogenase
-
 Syntrophus aciditrophicus
1.3.8.11 cyclohexanecarboxyl-CoA dehydrogenase
-
 Syntrophus aciditrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.10 FAD
-
 Syntrophus aciditrophicus
1.3.8.11 FAD
-
 Syntrophus aciditrophicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.8.11 0.049
-
 cyclohex-1-ene-1-carbonyl-CoA at pH 7.0 and 30°C Syntrophus aciditrophicus