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Literature summary extracted from

  • Martinez-Gomez, N.C.; Palmer, L.D.; Vivas, E.; Roach, P.L.; Downs, D.M.
    The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica (2011), J. Bacteriol., 193, 4582-4587.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B5
-
 Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.B5 C207S using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis Salmonella enterica
2.7.7.B5 C344S using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis Salmonella enterica
2.7.7.B5 C456S using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis Salmonella enterica
2.7.7.B5 D189A using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis Salmonella enterica
2.7.7.B5 K321M using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis Salmonella enterica
2.8.1.B3 C456S the mutant is not able to restore growth of a thiI mutant strain in minimal medium suggesting that C456 is required for thiazole synthesis in vivo Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B5 Salmonella enterica
-
-
-
2.8.1.B3 Salmonella enterica
-
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B5 ThiI bifunctional enzyme required for 4-thiouridine biosynthesis and thiamine synthesis. Adenylation activity for ThiI not required for thiazole synthesis Salmonella enterica
2.8.1.B3 ThiI
-
 Salmonella enterica

General Information

EC Number General Information Comment Organism
2.8.1.B3 metabolism ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety of thiamine. In 4-thiouridine biosynthesis, ThiI adenylates the tRNA uridine and transfers sulfur from a persulfide formed on the protein. The C-terminal rhodanese domain of ThiI is sufficient for thiazole synthesis in vivo Salmonella enterica