BRENDA - Enzyme Database

Effects of modified phycobilin biosynthesis in the cyanobacterium Synechococcus sp. strain PCC 7002

Alvey, R.M.; Biswas, A.; Schluchter, W.M.; Bryant, D.A.; J. Bacteriol. 193, 1663-1671 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.3.7.4
overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin, the cells show a phenotype only slightly less pigmented and blue-green than the wild-type, the strain producing phycobiliproteins carrying phytochromobilin grow much more slowly at low light intensity. Transformant colonies in which pcyA is inactivated in the HY2 overexpression background does not develop a chlorotic appearance, and segregation of the mutant and wild-type alleles is rapidly achieved
Arabidopsis thaliana
1.3.7.6
gene pebAB, overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin. Colonies of Synechococcus sp. strain PCC 7002 transformed with the pebAB overexpression cassette mostly exhibit a reddish-brown phenotype
Synechococcus sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.3.7.5
additional information
insertional inactivation of pcyA is not possible in wild-type Synechococcus sp. strain PCC 7002 due to lethality of the mutant, but in a variant heterologously expressing HY2 from Arabidopsis thaliana encoding a phytochromobilin:ferredoxin oxidoreductase
Synechococcus sp.
1.3.7.6
additional information
because PcyA, EC 1.3.7.5, and PebA utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light, phenotype, overview
Synechococcus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.7.4
biliverdin IXalpha + reduced ferredoxin
Arabidopsis thaliana
-
(3Z)-phytochromobilin + oxidized ferredoxin
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
Synechococcus sp.
-
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
Synechococcus sp. PCC 7335
-
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
Synechococcus sp.
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
Synechococcus sp. PCC 7335
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechococcus sp.
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechococcus sp. PCC 7335
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.7.4
Arabidopsis thaliana
-
-
-
1.3.7.5
Synechococcus sp.
-
-
-
1.3.7.6
Synechococcus sp.
-
gene pebAB
-
1.3.7.6
Synechococcus sp. PCC 7335
-
gene pebAB
-
1.14.15.20
Synechococcus sp.
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.7.4
biliverdin IXalpha + reduced ferredoxin
-
725252
Arabidopsis thaliana
(3Z)-phytochromobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
-
725252
Synechococcus sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha
725252
Synechococcus sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
-
725252
Synechococcus sp. PCC 7335
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha
725252
Synechococcus sp. PCC 7335
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
-
725252
Synechococcus sp.
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
(3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB
725252
Synechococcus sp.
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
-
725252
Synechococcus sp. PCC 7335
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
(3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB
725252
Synechococcus sp. PCC 7335
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
725252
Synechococcus sp.
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
725252
Synechococcus sp. PCC 7335
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.7.4
Ferredoxin
-
Arabidopsis thaliana
1.3.7.5
Ferredoxin
-
Synechococcus sp.
1.3.7.6
Ferredoxin
-
Synechococcus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.7.4
overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin, the cells show a phenotype only slightly less pigmented and blue-green than the wild-type, the strain producing phycobiliproteins carrying phytochromobilin grow much more slowly at low light intensity. Transformant colonies in which pcyA is inactivated in the HY2 overexpression background does not develop a chlorotic appearance, and segregation of the mutant and wild-type alleles is rapidly achieved
Arabidopsis thaliana
1.3.7.6
gene pebAB, overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin. Colonies of Synechococcus sp. strain PCC 7002 transformed with the pebAB overexpression cassette mostly exhibit a reddish-brown phenotype
Synechococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.7.4
Ferredoxin
-
Arabidopsis thaliana
1.3.7.5
Ferredoxin
-
Synechococcus sp.
1.3.7.6
Ferredoxin
-
Synechococcus sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.3.7.5
additional information
insertional inactivation of pcyA is not possible in wild-type Synechococcus sp. strain PCC 7002 due to lethality of the mutant, but in a variant heterologously expressing HY2 from Arabidopsis thaliana encoding a phytochromobilin:ferredoxin oxidoreductase
Synechococcus sp.
1.3.7.6
additional information
because PcyA, EC 1.3.7.5, and PebA utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light, phenotype, overview
Synechococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.7.4
biliverdin IXalpha + reduced ferredoxin
Arabidopsis thaliana
-
(3Z)-phytochromobilin + oxidized ferredoxin
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
Synechococcus sp.
-
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
Synechococcus sp. PCC 7335
-
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
Synechococcus sp.
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
Synechococcus sp. PCC 7335
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechococcus sp.
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechococcus sp. PCC 7335
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.7.4
biliverdin IXalpha + reduced ferredoxin
-
725252
Arabidopsis thaliana
(3Z)-phytochromobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
-
725252
Synechococcus sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha
725252
Synechococcus sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
-
725252
Synechococcus sp. PCC 7335
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.5
biliverdin Ixalpha + reduced ferredoxin
phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha
725252
Synechococcus sp. PCC 7335
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
-
725252
Synechococcus sp.
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
(3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB
725252
Synechococcus sp.
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
-
725252
Synechococcus sp. PCC 7335
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.3.7.6
biliverdin IXalpha + 2 reduced ferredoxin
(3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB
725252
Synechococcus sp. PCC 7335
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
725252
Synechococcus sp.
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
725252
Synechococcus sp. PCC 7335
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.3.7.4
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, overview
Arabidopsis thaliana
1.3.7.5
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview
Synechococcus sp.
1.3.7.5
malfunction
because PcyA and PebA, EC 1.3.7.6/1.3.7.3, utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light
Synechococcus sp.
1.3.7.5
physiological function
the pcyA gene of Synechococcus sp. strain PCC 7002 is essential
Synechococcus sp.
1.3.7.6
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview
Synechococcus sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.3.7.4
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, overview
Arabidopsis thaliana
1.3.7.5
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview
Synechococcus sp.
1.3.7.5
malfunction
because PcyA and PebA, EC 1.3.7.6/1.3.7.3, utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light
Synechococcus sp.
1.3.7.5
physiological function
the pcyA gene of Synechococcus sp. strain PCC 7002 is essential
Synechococcus sp.
1.3.7.6
evolution
synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview
Synechococcus sp.