BRENDA - Enzyme Database

Characterization of a bifunctional archaeal acyl coenzyme A carboxylase

Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.; J. Bacteriol. 185, 938-947 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
biotin
biotin-dependent enzyme
Acidianus brierleyi
6.4.1.2
palmitoyl-CoA
slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%)
Acidianus brierleyi
6.4.1.3
biotin
biotin-dependent enzyme
Acidianus brierleyi
6.4.1.3
palmitoyl-CoA
slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%)
Acidianus brierleyi
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.4.1.2
-
Acidianus brierleyi
6.4.1.3
-
Acidianus brierleyi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ATP
above 2.0 mM
Acidianus brierleyi
6.4.1.2
citrate
3 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.2
CoA
1 mM, 11-13% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
DTT
5-10 mM, 12% inhibition
Acidianus brierleyi
6.4.1.2
K+
-
Acidianus brierleyi
6.4.1.2
malonyl-CoA
0.5 mM, 50-57% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
methylmalonyl-CoA
0.5 mM, 79% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
Mg2+
above 4.0 mM
Acidianus brierleyi
6.4.1.2
Mn2+
above 2.0 mM
Acidianus brierleyi
6.4.1.2
Na+
-
Acidianus brierleyi
6.4.1.2
succinyl-CoA
0.5 mM, 84% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
sulfate
25 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.3
ATP
above 2.0 mM
Acidianus brierleyi
6.4.1.3
citrate
3 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.3
CoA
1 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
DTT
5-10 mM, 12% inhibition
Acidianus brierleyi
6.4.1.3
K+
-
Acidianus brierleyi
6.4.1.3
malonyl-CoA
0.5 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
methylmalonyl-CoA
0.5 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
Mg2+
above 4.0 mM
Acidianus brierleyi
6.4.1.3
Mn2+
above 2.0 mM
Acidianus brierleyi
6.4.1.3
Na+
-
Acidianus brierleyi
6.4.1.3
succinyl-CoA
0.5 mM, 57% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
sulfate
25 mM, more than 50% inhibition
Acidianus brierleyi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.17
-
acetyl-CoA
pH 7.5, 70°C
Acidianus brierleyi
6.4.1.3
0.1
-
propanoyl-CoA
pH 7.5, 70°C
Acidianus brierleyi
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Co2+
26% of the maximum activity is observed when Co2+ (10 to 15 mM) is used as a divalent cation
Acidianus brierleyi
6.4.1.2
Mg2+
maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM
Acidianus brierleyi
6.4.1.2
Mn2+
1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion
Acidianus brierleyi
6.4.1.3
Co2+
26% of the maximum activity is observed when Co2+(10 to 15 mM) is used as a divalent cation
Acidianus brierleyi
6.4.1.3
Mg2+
maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM
Acidianus brierleyi
6.4.1.3
Mn2+
1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion
Acidianus brierleyi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
20000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
59000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
62000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
540000
-
-
Acidianus brierleyi
6.4.1.3
20000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
59000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
62000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
540000
-
-
Acidianus brierleyi
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.2
Acidianus brierleyi
Q877I4 and Q877I3 and Q877I5
Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase
-
6.4.1.3
Acidianus brierleyi
Q877I4 and Q877I3 and Q877I5
Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.2
-
Acidianus brierleyi
6.4.1.3
-
Acidianus brierleyi
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
14.5
-
pH 7.5, 70°C, acetyl-CoA carboxylase activity
Acidianus brierleyi
6.4.1.3
11.9
-
pH 7.5, 70°C, propionyl-CoA carboxylase activity
Acidianus brierleyi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
the bifunctional enzyme also catalyzes the carboxylation of propionyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM)
725234
Acidianus brierleyi
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.2
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
the bifunctional enzyme also catalyzes the carboxylation of acetyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM)
725234
Acidianus brierleyi
ADP + phosphate + (S)-methylmalonyl-CoA
-
-
-
?
6.4.1.3
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
725234
Acidianus brierleyi
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.2
dodecamer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
dodecamer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
65
-
-
Acidianus brierleyi
6.4.1.2
70
-
assay at
Acidianus brierleyi
6.4.1.3
65
-
-
Acidianus brierleyi
6.4.1.3
70
-
assay at
Acidianus brierleyi
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.4.1.2
50
75
50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity
Acidianus brierleyi
6.4.1.3
50
75
50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity
Acidianus brierleyi
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.2
50
-
2 h, 40% loss of activity
Acidianus brierleyi
6.4.1.2
60
-
30 min, 50% loss of activity
Acidianus brierleyi
6.4.1.2
70
-
30 min, 90% loss of activity
Acidianus brierleyi
6.4.1.2
80
-
30 min, 95% loss of activity
Acidianus brierleyi
6.4.1.2
90
-
10 min, complete loss of activity
Acidianus brierleyi
6.4.1.3
50
-
2 h, 40% loss of activity
Acidianus brierleyi
6.4.1.3
60
-
30 min, 50% loss of activity
Acidianus brierleyi
6.4.1.3
70
-
30 min, 90% loss of activity
Acidianus brierleyi
6.4.1.3
80
-
30 min, 95% loss of activity
Acidianus brierleyi
6.4.1.3
90
-
10 min, complete loss of activity
Acidianus brierleyi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
6.4
6.9
at 70°C
Acidianus brierleyi
6.4.1.2
7.5
-
assay at
Acidianus brierleyi
6.4.1.3
6.4
6.9
at 70°C
Acidianus brierleyi
6.4.1.3
7.5
-
assay at
Acidianus brierleyi
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
biotin
biotin-dependent enzyme
Acidianus brierleyi
6.4.1.2
palmitoyl-CoA
slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%)
Acidianus brierleyi
6.4.1.3
biotin
biotin-dependent enzyme
Acidianus brierleyi
6.4.1.3
palmitoyl-CoA
slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%)
Acidianus brierleyi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
-
Acidianus brierleyi
6.4.1.3
-
Acidianus brierleyi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ATP
above 2.0 mM
Acidianus brierleyi
6.4.1.2
citrate
3 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.2
CoA
1 mM, 11-13% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
DTT
5-10 mM, 12% inhibition
Acidianus brierleyi
6.4.1.2
K+
-
Acidianus brierleyi
6.4.1.2
malonyl-CoA
0.5 mM, 50-57% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
methylmalonyl-CoA
0.5 mM, 79% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
Mg2+
above 4.0 mM
Acidianus brierleyi
6.4.1.2
Mn2+
above 2.0 mM
Acidianus brierleyi
6.4.1.2
Na+
-
Acidianus brierleyi
6.4.1.2
succinyl-CoA
0.5 mM, 84% inhibition of acetyl-CoA carboxylase
Acidianus brierleyi
6.4.1.2
sulfate
25 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.3
ATP
above 2.0 mM
Acidianus brierleyi
6.4.1.3
citrate
3 mM, more than 50% inhibition
Acidianus brierleyi
6.4.1.3
CoA
1 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
DTT
5-10 mM, 12% inhibition
Acidianus brierleyi
6.4.1.3
K+
-
Acidianus brierleyi
6.4.1.3
malonyl-CoA
0.5 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
methylmalonyl-CoA
0.5 mM, 60% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
Mg2+
above 4.0 mM
Acidianus brierleyi
6.4.1.3
Mn2+
above 2.0 mM
Acidianus brierleyi
6.4.1.3
Na+
-
Acidianus brierleyi
6.4.1.3
succinyl-CoA
0.5 mM, 57% inhibition of propionyl-CoA carboxylase
Acidianus brierleyi
6.4.1.3
sulfate
25 mM, more than 50% inhibition
Acidianus brierleyi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.17
-
acetyl-CoA
pH 7.5, 70°C
Acidianus brierleyi
6.4.1.3
0.1
-
propanoyl-CoA
pH 7.5, 70°C
Acidianus brierleyi
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Co2+
26% of the maximum activity is observed when Co2+ (10 to 15 mM) is used as a divalent cation
Acidianus brierleyi
6.4.1.2
Mg2+
maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM
Acidianus brierleyi
6.4.1.2
Mn2+
1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion
Acidianus brierleyi
6.4.1.3
Co2+
26% of the maximum activity is observed when Co2+(10 to 15 mM) is used as a divalent cation
Acidianus brierleyi
6.4.1.3
Mg2+
maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM
Acidianus brierleyi
6.4.1.3
Mn2+
1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion
Acidianus brierleyi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
20000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
59000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
62000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.2
540000
-
-
Acidianus brierleyi
6.4.1.3
20000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
59000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
62000
-
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
540000
-
-
Acidianus brierleyi
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
-
Acidianus brierleyi
6.4.1.3
-
Acidianus brierleyi
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
14.5
-
pH 7.5, 70°C, acetyl-CoA carboxylase activity
Acidianus brierleyi
6.4.1.3
11.9
-
pH 7.5, 70°C, propionyl-CoA carboxylase activity
Acidianus brierleyi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
the bifunctional enzyme also catalyzes the carboxylation of propionyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM)
725234
Acidianus brierleyi
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.2
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA
725234
Acidianus brierleyi
?
-
-
-
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
the bifunctional enzyme also catalyzes the carboxylation of acetyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM)
725234
Acidianus brierleyi
ADP + phosphate + (S)-methylmalonyl-CoA
-
-
-
?
6.4.1.3
additional information
neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme
725234
Acidianus brierleyi
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.2
dodecamer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
6.4.1.3
dodecamer
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE
Acidianus brierleyi
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
65
-
-
Acidianus brierleyi
6.4.1.2
70
-
assay at
Acidianus brierleyi
6.4.1.3
65
-
-
Acidianus brierleyi
6.4.1.3
70
-
assay at
Acidianus brierleyi
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.4.1.2
50
75
50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity
Acidianus brierleyi
6.4.1.3
50
75
50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity
Acidianus brierleyi
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.4.1.2
50
-
2 h, 40% loss of activity
Acidianus brierleyi
6.4.1.2
60
-
30 min, 50% loss of activity
Acidianus brierleyi
6.4.1.2
70
-
30 min, 90% loss of activity
Acidianus brierleyi
6.4.1.2
80
-
30 min, 95% loss of activity
Acidianus brierleyi
6.4.1.2
90
-
10 min, complete loss of activity
Acidianus brierleyi
6.4.1.3
50
-
2 h, 40% loss of activity
Acidianus brierleyi
6.4.1.3
60
-
30 min, 50% loss of activity
Acidianus brierleyi
6.4.1.3
70
-
30 min, 90% loss of activity
Acidianus brierleyi
6.4.1.3
80
-
30 min, 95% loss of activity
Acidianus brierleyi
6.4.1.3
90
-
10 min, complete loss of activity
Acidianus brierleyi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
6.4
6.9
at 70°C
Acidianus brierleyi
6.4.1.2
7.5
-
assay at
Acidianus brierleyi
6.4.1.3
6.4
6.9
at 70°C
Acidianus brierleyi
6.4.1.3
7.5
-
assay at
Acidianus brierleyi