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Literature summary extracted from

  • Rivera-Perez, C.; Nouzova, M.; Clifton, M.E.; Garcia, E.M.; Leblanc, E.; Noriega, F.G.
    Aldehyde dehydrogenase 3 converts farnesal into farnesoic acid in the corpora allata of mosquitoes (2013), Insect Biochem. Mol. Biol., 43, 675-682.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.94 farnesal massive accumulation of the potentially toxic farnesal can stimulate the activity of a farnesal reductase or another enzyme that could convert farnesal back into farnesol Aedes aegypti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.94 expressed in Escherichia coli Aedes aegypti
1.2.1.94 five recombinant His-tagged AaALDH3s, representing AaALDH3-2 and the 4 splice variants of AaALDH3-1, PA, PB, PC, PD, overexpressed in Escherichia coli Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.94 (2E,6E)-farnesal + NAD+ Aedes aegypti NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid (2E,6E)-farnesoate + NADH + 2 H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 (2E,6E)-farnesal + NAD+ Aedes aegypti Rockefeller NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid (2E,6E)-farnesoate + NADH + 2 H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.94 Aedes aegypti
-
-
-
1.2.1.94 Aedes aegypti
-
 Dengue mosquito
-
1.2.1.94 Aedes aegypti Rockefeller
-
 Dengue mosquito
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.94 corpora allata
-
 Aedes aegypti
-
1.2.1.94 corpus allatum presents the five transcripts, with their relative proportions changing during development Aedes aegypti
-
1.2.1.94 hindgut AaALDH3-1-RC transcript abundance, quantified by qPCR Aedes aegypti
-
1.2.1.94 malpighian tubule AaALDH3-1-RB transcript abundance, quantified by qPCR Aedes aegypti
-
1.2.1.94 additional information AaALDH3-2 gene has comparatively lower levels of transcript Aedes aegypti
-
1.2.1.94 nervous system AaALDH3-1-RD transcript abundance, quantified by qPCR Aedes aegypti
-
1.2.1.94 ovary AaALDH3-1-RA transcript abundance, quantified by qPCR Aedes aegypti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.94 additional information
-
 no activity of AaALDH3-1-PA with decanal, no activity of AaALDH3-1-PA, AaALDH3-1-PD, and AaALDH3-2 with NADP+ Aedes aegypti
1.2.1.94 7.08
-
 recombinant AaALDH3-1-PB with farnesal and NADP+ as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 7.13
-
 recombinant AaALDH3-1-PC with farnesal and NADP+ as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 103.7
-
 recombinant AaALDH3-1-PD with decanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 116.8
-
 recombinant AaALDH3-1-PB with octanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 132.5
-
 recombinant AaALDH3-2 with decanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 152.7
-
 recombinant AaALDH3-1-PA with octanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 168.1
-
 recombinant AaALDH3-1-PD with octanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 203.9
-
 recombinant AaALDH3-2 with octanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 209.2
-
 recombinant AaALDH3-2 with farnesal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 214.6
-
 recombinant AaALDH3-1-PC with octanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 268
-
 recombinant AaALDH3-1-PD with farnesal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 269.1
-
 recombinant AaALDH3-1-PB with farnesal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 272.9
-
 recombinant AaALDH3-1-PA with farnesal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 287.1
-
 recombinant AaALDH3-1-PB with decanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 362.4
-
 recombinant AaALDH3-1-PC with decanal as substrate, pH 9.5 and 37°C Aedes aegypti
1.2.1.94 468.2
-
 recombinant AaALDH3-1-PC with farnesal as substrate, pH 9.5 and 37°C Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.94 (2E,6E)-farnesal + NAD+ NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid Aedes aegypti (2E,6E)-farnesoate + NADH + 2 H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 (2E,6E)-farnesal + NAD+ NADP+ can not substitute NAD+, AaALDH3-1-PC has the highest activity with farnesal, AaALDH3-1 is mainly responsible for the conversion of farnesal to farnesoic acid Aedes aegypti Rockefeller (2E,6E)-farnesoate + NADH + 2 H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 (2E,6E)-farnesal + NAD+ + H2O
-
 Aedes aegypti (2E,6E)-farnesoic acid + NADH + H+
-
 ?
1.2.1.94 decanal + NAD+ + H2O
-
 Aedes aegypti decanoic acid + NADH + H+
-
 ?
1.2.1.94 decanal + NAD+ + H2O
-
 Aedes aegypti decanoic acid + NADH + H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 decanal + NAD+ + H2O
-
 Aedes aegypti Rockefeller decanoic acid + NADH + H+
-
 ?
1.2.1.94 decanal + NAD+ + H2O
-
 Aedes aegypti Rockefeller decanoic acid + NADH + H+ reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 additional information no activity with NADP+ Aedes aegypti ?
-
 ?
1.2.1.94 additional information no activity with NADP+ Aedes aegypti Rockefeller ?
-
 ?
1.2.1.94 octanal + NAD+
-
 Aedes aegypti octanoic acid + NADH reaction products analyzed by LC-MS and identified based on the diagnostic ions ?
1.2.1.94 octanal + NAD+ + H2O
-
 Aedes aegypti octanoic acid + NADH + H+
-
 ?
1.2.1.94 octanal + NAD+ + H2O
-
 Aedes aegypti Rockefeller octanoic acid + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.94 AaALDH3-1
-
 Aedes aegypti
1.2.1.94 aldehyde dehydrogenase 3
-
 Aedes aegypti
1.2.1.94 ALDH3
-
 Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.94 NAD+
-
 Aedes aegypti
1.2.1.94 NAD+ could not be substituted by NADP+ Aedes aegypti

Expression

EC Number Organism Comment Expression
1.2.1.94 Aedes aegypti injection of dsRNAs resulted in a approx. 80% significant reduction of AaALDH3-1 and AaALDH3-2 mRNAs, treatment with dsRNA for the AaALDH3-1 gene results in stronger reductions of farnesoic acid and juvenile hormone than treatment with dsRNA for the AaALDH3-2 gene down
1.2.1.94 Aedes aegypti in the presence of an excess of farnesal and NAD+, highly active glands produces 10 times more farnesoic acid than suppressed glands, 24 h after blood feeding up

General Information

EC Number General Information Comment Organism
1.2.1.94 physiological function results suggest that in the female Dengue mosquito Aedes aegypti, AaALDH3 enzyme plays a critical role in the regulation of juvenile hormone biosynthesis after blood feeding Aedes aegypti
1.2.1.94 physiological function the enzyme plays a key role in the regulation of juvenile hormone synthesis in blood-fed mosquito females Aedes aegypti