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Literature summary extracted from
- Overexpression and biochemical characterization of soluble pyridine nucleotide transhydrogenase from Escherichia coli (2011), FEMS Microbiol. Lett., 320, 9-14.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | ADP | 75.5% activation at 2 mM | Escherichia coli |  |
| 1.6.1.1 | ADP | 175.5% activity at 2 mM ADP | Escherichia coli | |
| 1.6.1.1 | AMP | 71.8% activation at 2 mM | Escherichia coli | |
| 1.6.1.1 | AMP | 171.8% activity at 2 mM AMP | Escherichia coli | |
| 1.6.1.1 | ATP | 53.4% activation at 2 mM | Escherichia coli | |
| 1.6.1.1 | ATP | 153.4% activity at 2 mM ATP | Escherichia coli | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.1.1 | expressed in Escherichia coli DH5alpha cells | Escherichia coli |
| 1.6.1.1 | expression of the soluble enzyme in Escherichia coli strain DH5alpha | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | 2-mercaptoethanol | - |
Escherichia coli | |
| 1.6.1.1 | beta-mercaptoethanol | 72% residual activity at 0.2% (v/v) | Escherichia coli | |
| 1.6.1.1 | Ca2+ | 91.2% residual activity at 2 mM; slightly inhibitory | Escherichia coli | |
| 1.6.1.1 | Co2+ | - |
Escherichia coli | |
| 1.6.1.1 | Cu2+ | complete inhibition at 2 mM | Escherichia coli | |
| 1.6.1.1 | dithiothreitol | 75.3% residual activity at 2 mM | Escherichia coli | |
| 1.6.1.1 | DMSO | slightly inhibitory | Escherichia coli | |
| 1.6.1.1 | DTT | - |
Escherichia coli | |
| 1.6.1.1 | EDTA | 72.6% residual activity at 2 mM | Escherichia coli | |
| 1.6.1.1 | Mn2+ | 67% residual activity at 2 mM | Escherichia coli | |
| 1.6.1.1 | additional information | no or poor inhibition of the enzyme by Na+, Rb+, K+, Li+,, and Mg2+; the enzyme is not inhibited by thio-NAD+ and DMSO | Escherichia coli | |
| 1.6.1.1 | NADPH | EcSTH activity is strongly inhibited by excess NADPH, but not by thio-NAD+; the enzyme is strongly inhibited by excess NADPH | Escherichia coli | |
| 1.6.1.1 | Ni2+ | 7.4% residual activity at 2 mM | Escherichia coli | |
| 1.6.1.1 | Zn2+ | 10.1% residual activity at 2 mM | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.1.1 | 0.06829 | - |
NADPH | at pH 7.5 and 35°C | Escherichia coli | |
| 1.6.1.1 | 0.0683 | - |
NADPH | pH 7.5, 35°C | Escherichia coli | |
| 1.6.1.1 | 0.1332 | - |
thio-NAD+ | pH 7.5, 35°C | Escherichia coli | |
| 1.6.1.1 | 0.1332 | - |
thio-NAD+ | at pH 7.5 and 35°C | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.1.1 | soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | additional information | not influenced by Na+, Rb+, K+, Li+, and Mg2+ | Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 51500 | - |
x * 51500, calculated from amino acid sequence | Escherichia coli |
| 1.6.1.1 | 52000 | - |
x * 52000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.1 | NADPH + NAD+ | Escherichia coli | - |
NADP+ + NADH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.1.1 | Escherichia coli | - |
- |
- |
| 1.6.1.1 | Escherichia coli | P27306 | substrain MG1655, gene sth | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.1.1 | TALON metal affinity resin column chromatography | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.6.1.1 | 25°C, purified enzyme, 25 days, 65% activity remaining | Escherichia coli |
| 1.6.1.1 | 4°C, purified enzyme, 25 days, stable | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.1 | NADPH + NAD+ | - |
Escherichia coli | NADP+ + NADH | - |
r | |
| 1.6.1.1 | NADPH + thio-NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + thio-NADH + H+[side 2] | - |
r | |
| 1.6.1.1 | NADPH + thio-NAD+ + H+[side 1] | EcSTH has a 1.25fold preference for NADPH over thio-NAD+ | Escherichia coli | NADP+ + thio-NADH + H+[side 2] | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.1.1 | ? | x * 52000, SDS-PAGE | Escherichia coli |
| 1.6.1.1 | ? | x * 51500, calculated from amino acid sequence | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.1.1 | energy-independent soluble pyridine nucleotide transhydrogenase | - |
Escherichia coli |
| 1.6.1.1 | soluble pyridine nucleotide transhydrogenase | - |
Escherichia coli |
| 1.6.1.1 | STH | - |
Escherichia coli |
| 1.6.1.1 | UdhA | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 35 | - |
- |
Escherichia coli |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 20 | 45 | more than 70% activity between 20 and 45°C | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 4 | 50 | the enzyme retains 50% activity after 5 h at 50°C. The enzyme is stable at 4°C for 25 days and retains 65% activity at 25°C. The enzyme is rapidly inactivated at high temperatures, retaining 80%, 50% and 10% activity after incubation for 30 min at 50, 57 and 62°C, respectively | Escherichia coli |
| 1.6.1.1 | 50 | - |
purified enzyme, pH 7.5, 5h, 50% activity remaining, 80% activity remaining after 30 min | Escherichia coli |
| 1.6.1.1 | 57 | - |
purified enzyme, pH 7.5, 30 min, 50% activity remaining | Escherichia coli |
| 1.6.1.1 | 62 | - |
purified enzyme, pH 7.5, 30 min, 10% activity remaining | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.1.1 | 167.9 | - |
NADPH | pH 7.5, 35°C | Escherichia coli | |
| 1.6.1.1 | 167.9 | - |
NADPH | at pH 7.5 and 35°C | Escherichia coli | |
| 1.6.1.1 | 259.5 | - |
thio-NAD+ | pH 7.5, 35°C | Escherichia coli | |
| 1.6.1.1 | 259.5 | - |
thio-NAD+ | at pH 7.5 and 35°C | Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 7.5 | - |
- |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 7 | 9 | more than 50% activity between pH 7.0 and 9.0 | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | flavin | flavoprotein | Escherichia coli | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.6.1.1 | evolution | two pyridine nucleotide transhydrogenases: the energy-independent soluble pyridine nucleotide transhydrogenase (STH or UdhA) (EC 1.6.1.1) and the membrane-bound, energy-dependent pyridine nucleotide transhydrogenase (TH or PntAB) (EC 1.6.1.2). PntAB is widely distributed in the mitochondria and some bacteria, while STH is found only in certain Gammaproteobacteria and gram-positive bacteria | Escherichia coli |
| 1.6.1.1 | physiological function | the soluble pyridine nucleotide transhydrogenase, STH, is an energy-independent flavoprotein that directly catalyzes hydride transfer between NAD(H) and NADP(H) to maintain homeostasis of these two redox cofactors | Escherichia coli |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.1.1 | 1940 | - |
thio-NAD+ | at pH 7.5 and 35°C | Escherichia coli | |
| 1.6.1.1 | 2460 | - |
NADPH | at pH 7.5 and 35°C | Escherichia coli | |
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