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Literature summary extracted from

  • Gadda, G.; Fitzpatrick, P.F.
    Solvent isotope and viscosity effects on the steady-state kinetics of the flavoprotein nitroalkane oxidase (2013), FEBS Lett., 587, 2785-2789.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.1 Fusarium oxysporum Q8X1D8
-
-
1.7.3.1 Fusarium oxysporum ATCC 695 Q8X1D8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.1
-
 Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.1 nitroethane + H2O solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat Fusarium oxysporum ethanal + nitrite + H2O2
-
 ?
1.7.3.1 nitroethane + H2O solvent isotope and viscositiy effects: the kcat proton inventory is consistent with a single exchangeable proton in flight, while the kcat/KM is consistent with either a single proton in flight in the transition state or a medium effect. Increasing the solvent viscosity does not affect the kcat or kcat/KM value significantly, establishing that nitroethane binding is at equilibrium and that product release does not limit kcat Fusarium oxysporum ATCC 695 ethanal + nitrite + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.3.1 2.4
-
 nitroethane cosubstrate D2O, 30°C, pH 8.5 Fusarium oxysporum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.3.1 0.6
-
 nitroethane cosubstrate D2O, 30°C, pH 8.5 Fusarium oxysporum