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Literature summary extracted from

  • Serrano, G.C.; Rezende e Silva Figueira, T.; Kiyota, E.; Zanata, N.; Arruda, P.
    Lysine degradation through the saccharopine pathway in bacteria: LKR and SDH in bacteria and its relationship to the plant and animal enzymes (2012), FEBS Lett., 586, 905-911.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.9 expressed in Escherichia coli BL21(DE3) cells Nostoc punctiforme
1.5.1.9 expressed in Escherichia coli BL21(DE3) cells Ruegeria pomeroyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.9 44000
-
 x * 44000, calculated from amino acid sequence Nostoc punctiforme
1.5.1.9 44000
-
 x * 44000, calculated from amino acid sequence Ruegeria pomeroyi
1.5.1.9 44100
-
 x * 44100, SDS-PAGE Nostoc punctiforme
1.5.1.9 44100
-
 x * 44100, SDS-PAGE Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.9 saccharopine + NAD+ + H2O Nostoc punctiforme
-
 L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r
1.5.1.9 saccharopine + NAD+ + H2O Ruegeria pomeroyi
-
 L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r
1.5.1.9 saccharopine + NAD+ + H2O Nostoc punctiforme ATCC 29133
-
 L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.9 Nostoc punctiforme
-
-
-
1.5.1.9 Nostoc punctiforme ATCC 29133
-
-
-
1.5.1.9 Ruegeria pomeroyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.9 Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Nostoc punctiforme
1.5.1.9 Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.9 saccharopine + NAD+ + H2O
-
 Nostoc punctiforme L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r
1.5.1.9 saccharopine + NAD+ + H2O
-
 Ruegeria pomeroyi L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r
1.5.1.9 saccharopine + NAD+ + H2O
-
 Nostoc punctiforme ATCC 29133 L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.9 ? x * 44000, calculated from amino acid sequence Nostoc punctiforme
1.5.1.9 ? x * 44000, calculated from amino acid sequence Ruegeria pomeroyi
1.5.1.9 ? x * 44100, SDS-PAGE Nostoc punctiforme
1.5.1.9 ? x * 44100, SDS-PAGE Ruegeria pomeroyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.9 saccharopine dehydrogenase
-
 Nostoc punctiforme
1.5.1.9 saccharopine dehydrogenase
-
 Ruegeria pomeroyi
1.5.1.9 SDH
-
 Nostoc punctiforme
1.5.1.9 SDH
-
 Ruegeria pomeroyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.9 NAD+ the enzyme has slightly higher affinity for NAD+ compared to NADP+ Nostoc punctiforme
1.5.1.9 NAD+ the enzyme has slightly higher affinity for NAD+ compared to NADP+ Ruegeria pomeroyi
1.5.1.9 NADP+
-
 Nostoc punctiforme
1.5.1.9 NADP+
-
 Ruegeria pomeroyi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.9 Nostoc punctiforme calculated from amino acid sequence
-
 5
1.5.1.9 Ruegeria pomeroyi calculated from amino acid sequence
-
 5