EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.40 | overexpression in Escherichia coli | Methanothermobacter thermautotrophicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.1.40 | hanging drop method, 4°C | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.40 | 0.05 | - |
NADPH | pH 6.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
1.5.1.40 | 0.07 | - |
NADP+ | pH 8.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
1.5.1.40 | 0.15 | - |
reduced coenzyme F420 | pH 8.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
1.5.1.40 | 0.3 | - |
oxidized coenzyme F420 | pH 6.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 23500 | - |
4 * 23500, calculated from sequence | Methanothermobacter thermautotrophicus |
1.5.1.40 | 28000 | - |
4 * 28000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.5.1.40 | 110000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.40 | Methanothermobacter thermautotrophicus | D9PVP5 | - |
- |
1.5.1.40 | Methanothermobacter thermautotrophicus DSM 2133 | D9PVP5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.40 | - |
Methanothermobacter thermautotrophicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.40 | 4°C, purified enzyme at a protein concentration of 20 mg/ml is completely stable when stored at 4°C in 50 mM Tricine/KOH pH 8.0 containing 0.2 M KCl | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | oxidized coenzyme F420 + NADPH + H+ | - |
Methanothermobacter thermautotrophicus | reduced coenzyme F420 + NADP+ | - |
? | |
1.5.1.40 | oxidized coenzyme F420 + NADPH + H+ | - |
Methanothermobacter thermautotrophicus DSM 2133 | reduced coenzyme F420 + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.40 | homotetramer | 4 * 28000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.5.1.40 | homotetramer | 4 * 23500, calculated from sequence | Methanothermobacter thermautotrophicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.40 | F420H2:NADP oxidoreductase | - |
Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 3.5 | - |
reduction of oxidized coenzyme F420 with NADPH | Methanothermobacter thermautotrophicus |
1.5.1.40 | 8 | - |
reduction of NADP+ with reduced F420H2 | Methanothermobacter thermautotrophicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.5.1.40 | Methanothermobacter thermautotrophicus | calculated from sequence | - |
4.6 |