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Literature summary extracted from

  • Hayakawa, S.; Matsumura, H.; Nakamura, N.; Yohda, M.; Ohno, H.
    Identification of the rate-limiting step of the peroxygenase reactions catalyzed by the thermophilic cytochrome P450 from Sulfolobus tokodaii strain 7 (2014), FEBS J., 281, 1409-1416.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.11.2.1 F310A/A320Q specific activity of peroxygenase is significantly increased over a broad pH-range. The pH dependence of the reaction is altered to bell shaped, with maximum activities near pH 5. At pH 5, the mutant is over 15 times more active than wild-type P450st Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 7.2
-
 H2O2 pH 5, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 9.3
-
 H2O2 pH 10, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 23.2
-
 H2O2 pH 5, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 26.2
-
 H2O2 pH 10, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 30.6
-
 H2O2 pH 7, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 81.4
-
 H2O2 pH 7, 30°C, wild-type enzyme Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Sulfurisphaera tokodaii
-
-
-
1.11.2.1 Sulfurisphaera tokodaii 7
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 ethylbenzene + H2O2
-
 Sulfurisphaera tokodaii 1-phenylethanol + H2O 1-phenylethanol is the major product ?
1.11.2.1 ethylbenzene + H2O2
-
 Sulfurisphaera tokodaii 7 1-phenylethanol + H2O 1-phenylethanol is the major product ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 P450st
-
 Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 0.07
-
 H2O2 pH 5, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 0.09
-
 H2O2 pH 5, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.09
-
 H2O2 pH 10, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 0.13
-
 H2O2 pH 7, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.16
-
 H2O2 pH 10, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.34
-
 H2O2 pH 7, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 7
-
-
 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.2.1 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 45% of maximal activity Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 0.0016
-
 H2O2 pH 7, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.004
-
 H2O2 pH 5, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.006
-
 H2O2 pH 10, 30°C, wild-type enzyme Sulfurisphaera tokodaii
1.11.2.1 0.0096
-
 H2O2 pH 10, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 0.0097
-
 H2O2 pH 5, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii
1.11.2.1 0.011
-
 H2O2 pH 7, 30°C, mutant enzyme F310A/F320Q Sulfurisphaera tokodaii