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Literature summary extracted from

  • Sutak, R.; Hrdy, I.; Dolezal, P.; Cabala, R.; Sedinova, M.; Lewin, J.; Harant, K.; Mueller, M.; Tachezy, J.
    Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis (2012), FEBS J., 279, 2768-2780.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.80 2,2'-dipyridyl 5 mM, 80% residual activity Trichomonas vaginalis
1.1.1.80 4-hydroxymercuribenzoic acid 0.1 mM, 49% residual activity Trichomonas vaginalis
1.1.1.80 4-Methylpyrazole 5 mM, 68% residual activity Trichomonas vaginalis
1.1.1.80 iodacetamide 50 mM, 49% residual activity Trichomonas vaginalis
1.1.1.80 additional information not inhibitory: EDTA up to 5 mM Trichomonas vaginalis
1.1.1.80 pyrazole 5 mM, 74% residual activity Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.80 0.000061
-
 Butanone 37°C, pH 7.0 Trichomonas vaginalis
1.1.1.80 0.000093
-
 acetone 37°C, pH 7.0 Trichomonas vaginalis
1.1.1.80 0.000256
-
 pentan-3-one 37°C, pH 7.0 Trichomonas vaginalis
1.1.1.80 0.00028
-
 acetaldehyde 37°C, pH 7.0 Trichomonas vaginalis
1.1.1.80 0.00056
-
 propan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.00078
-
 butan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.0015
-
 pentan-3-ol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.0052
-
 NADP+ pH 7.0, 37°C, cosubstrate acetone Trichomonas vaginalis
1.1.1.80 0.0056
-
 pentan-2-ol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.0275
-
 propan-1-ol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.1
-
 ethanol 37°C, pH 9.0 Trichomonas vaginalis
1.1.1.80 0.51
-
 NADPH pH 9.0, 37°C, cosubstrate propan-2-ol Trichomonas vaginalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.80 cytosol
-
 Trichomonas vaginalis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.80 39100
-
 2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis
1.1.1.80 43000
-
 2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis
1.1.1.80 81000
-
 gel filtration Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.80 butan-2-ol + NADP+ Trichomonas vaginalis preferred direction of the reaction is reduction rather than oxidation butanone + NADPH + H+
-
 r
1.1.1.80 propan-2-ol + NADP+ Trichomonas vaginalis preferred direction of the reaction is reduction rather than oxidation acetone + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.80 Trichomonas vaginalis A2DJ40 putative
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.80
-
 Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.80 acetaldehyde + NADPH + H+
-
 Trichomonas vaginalis ethanol + NADP+
-
 r
1.1.1.80 acetone + NADPH + H+
-
 Trichomonas vaginalis propan-2-ol + NADP+
-
 r
1.1.1.80 butan-2-ol + NADP+ preferred direction of the reaction is reduction rather than oxidation Trichomonas vaginalis butanone + NADPH + H+
-
 r
1.1.1.80 butanone + NADPH + H+
-
 Trichomonas vaginalis butan-2-ol + NADP+
-
 r
1.1.1.80 ethanol + NADP+
-
 Trichomonas vaginalis acetaldehyde + NADPH + H+
-
 r
1.1.1.80 pentan-2-ol + NADP+
-
 Trichomonas vaginalis pentan-2-one + NADPH + H+
-
 r
1.1.1.80 pentan-3-ol + NADP+
-
 Trichomonas vaginalis pentan-3-one + NADPH + H+
-
 r
1.1.1.80 pentan-3-one + NADPH + H+
-
 Trichomonas vaginalis pentan-3-ol + NADP+
-
 r
1.1.1.80 propan-1-ol + NADP+
-
 Trichomonas vaginalis propanal + NADPH + H+
-
 r
1.1.1.80 propan-2-ol + NADP+ preferred direction of the reaction is reduction rather than oxidation Trichomonas vaginalis acetone + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.80 dimer 2 * 43000, SDS-PAGE, 2 * 39100, calculated Trichomonas vaginalis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.80 S-ADH
-
 Trichomonas vaginalis
1.1.1.80 TVAG_228780
-
 Trichomonas vaginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.80 5.5
-
 reduction of acetone, citrate buffer Trichomonas vaginalis
1.1.1.80 6.5 7.5 reduction of acetone, Bis/Tris propane buffer Trichomonas vaginalis
1.1.1.80 8 8.5 oxidation of propan-2-ol, Bis/Tris propane buffer Trichomonas vaginalis
1.1.1.80 9.5
-
 oxidation of propan-2-ol, glycine/HCl buffer Trichomonas vaginalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.80 NADPH strictly specific for NADPH Trichomonas vaginalis

Expression

EC Number Organism Comment Expression
1.1.1.80 Trichomonas vaginalis iron limitation results in a 1.5fold increase in enzyme mRNA up