EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.18 | expressed in Pichia pastoris X-33 cells | Thermothelomyces myriococcoides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.035 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.085 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.09 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.71 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 6.75 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 387 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Thermothelomyces myriococcoides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | ammonium sulfate precipitation and phenyl Sepharose column chromatography | Thermothelomyces myriococcoides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 0.2 | - |
culture supernatant, in 100 mM sodium acetate buffer, pH 5.0, at 30°C | Thermothelomyces myriococcoides |
1.1.99.18 | 3.1 | - |
after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30°C | Thermothelomyces myriococcoides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | cellotetraose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichlorophenol indophenol | weak substrate | Thermothelomyces myriococcoides | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | lactose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | maltose + oxidized 2,6-dichlorophenolindophenol | - |
Thermothelomyces myriococcoides | 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | maltotetraose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | maltotriose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | ? + reduced 2,6-dichlorophenol indophenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.18 | Cdh | - |
Thermothelomyces myriococcoides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.315 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.48 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 2.7 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 4.11 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 4.68 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 4.8 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.012 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.047 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.676 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 32.12 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 52 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 117.1 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |