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Literature summary extracted from

  • Ritter, D.W.; Roberts, J.R.; McShane, M.J.
    Glycosylation site-targeted PEGylation of glucose oxidase retains native enzymatic activity (2013), Enzyme Microb. Technol., 52, 279-285.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis the enzyme is useful as biosensor for glucose detection Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.4 additional information PEGylation of GOx provides stability against denaturation or hydrolytic cleavage, glycosylation site-targeted PEGylation of glucose oxidase retains native enzymatic activity, bioconjugate's potential of the enzyme in an optical biosensing assay, overview. The bioconjugate is entrapped within a poly(2-hydroxyethyl methacrylate) hydrogel containing an oxygen-sensitive phosphor, and the construct is shown to respond approximately linearly over the physiologically-relevant glucose range, overview Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 86400
-
 2 * 86400, native enzyme, SDS-PAGE Aspergillus niger
1.1.3.4 140000
-
 native enzyme, gel filtration Aspergillus niger
1.1.3.4 182200
-
 x * 182200, PEGylated enzyme, SDS-PAGE Aspergillus niger
1.1.3.4 669000
-
 PEGylated enzyme, gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 commercial preparation type VII Aspergillus niger
-

Subunits

EC Number Subunits Comment Organism
1.1.3.4 ? x * 182200, PEGylated enzyme, SDS-PAGE Aspergillus niger
1.1.3.4 dimer 2 * 86400, native enzyme, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 GOX
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 35
-
 assay at Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 37
-
 loss in specific activity of PEGylated GOx and native GOx in the absence of glucose and at 37°C, the PEGylated enzyme is more stable and retains 62.6% after 24 h and about 40% after 30 days, the native enzyme retains 67.3% after 24 h and about 20% after 30 days, overview Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.1
-
 assay at Aspergillus niger