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Literature summary extracted from

  • Yang, Y.; Lu, L.; Gao, F.; Zhao, Y.
    Characterization of an efficient catalytic and organic solvent-tolerant azoreductase toward methyl red from Shewanella oneidensis MR-1 (2013), Environ. Sci. Pollut. Res. Int., 20, 3232-3239.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 expression in Escherichia coli Shewanella oneidensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Zn2+ 5 mM, 30% residual activity Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0028
-
 Methyl red cosubstrate NADPH, pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 0.0092
-
 Methyl red cosubstrate NADH, pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 0.48
-
 NADH pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 0.941
-
 NADPH pH 6.5, 25°C Shewanella oneidensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.6 Mg2+ activation, optimum concentration 25 mM giving 126% of initial activity Shewanella oneidensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 27000
-
 x * 27000, SDS-PAGE Shewanella oneidensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.7.1.6 Acetone 30%, no significant loss of activity Shewanella oneidensis
1.7.1.6 dimethyl sulfoxide 30%, no significant loss of activity Shewanella oneidensis
1.7.1.6 Ethanol 20%, no significant loss of activity Shewanella oneidensis
1.7.1.6 isopropanol 20%, no significant loss of activity Shewanella oneidensis
1.7.1.6 Methanol 30%, no significant loss of activity Shewanella oneidensis
1.7.1.6 propanol 10%, no significant loss of activity Shewanella oneidensis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Shewanella oneidensis Q8E990
-
-
1.7.1.6 Shewanella oneidensis MR-1 / ATCC 700550 Q8E990
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 156.1
-
 pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 184.6
-
 presence of 25 microM FMN, pH 6.5, 25°C Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 methyl red + NADH + H+
-
 Shewanella oneidensis ? + NAD+
-
 ?
1.7.1.6 methyl red + NADH + H+
-
 Shewanella oneidensis MR-1 / ATCC 700550 ? + NAD+
-
 ?
1.7.1.6 methyl red + NADPH + H+
-
 Shewanella oneidensis ? + NADP+
-
 ?
1.7.1.6 methyl red + NADPH + H+
-
 Shewanella oneidensis MR-1 / ATCC 700550 ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 ? x * 27000, SDS-PAGE Shewanella oneidensis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoR
-
 Shewanella oneidensis
1.7.1.6 SO 4396
-
 Shewanella oneidensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 6.5
-
-
 Shewanella oneidensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.6 5.5 7.5 more than 50% of maximum activity Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN
-
 Shewanella oneidensis
1.7.1.6 NADH preferred cofactor Shewanella oneidensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.6 30
-
 NADPH pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 210
-
 NADH pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 6760
-
 Methyl red cosubstrate NADPH, pH 6.5, 25°C Shewanella oneidensis
1.7.1.6 8970
-
 Methyl red cosubstrate NADH, pH 6.5, 25°C Shewanella oneidensis