Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vagbo, C.B.; Svaasand, E.K.; Aas, P.A.; Krokan, H.E.
    Methylation damage to RNA induced in vivo in Escherichia coli is repaired by endogenous AlkB as part of the adaptive response (2013), DNA Repair, 12, 188-195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 AlkB overexpression in Escherichia coli AlkB-deficient strains HK82 and BW25113. AlkB mutant strain HK82 is transformed with the low-copy-number expression plasmid pJB658 containing the alkB gene following a toluic acid-inducible promoter. The recombinant AlkB expressed is less active than the natural endogenous form Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli
-
 DNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 DNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N1-methyladenine + 2-oxoglutarate + O2 Escherichia coli
-
 adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N1-methyladenine + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N3-methylcytosine + 2-oxoglutarate + O2 Escherichia coli
-
 cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N3-methylcytosine + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli
-
 RNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli AB1157
-
 RNA-base + formaldehyde + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 Escherichia coli AB1157
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli DNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 DNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 N3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli RNA-base + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli AB1157 RNA-base + formaldehyde + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 AlkB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.33 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.33 7
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
1.14.11.33 malfunction an AlkB-deficient strain accumulates N1-methyladenine at a high rate Escherichia coli
1.14.11.33 additional information the recombinant AlkB expressed is less active than the natural endogenous form Escherichia coli
1.14.11.33 physiological function protein AlkB repairs cytotoxic 1-methyladenine and 3-methylcytosine lesions induced by methyl methanesulfonate in DNA and RNA in vitro and in pre-damaged DNA and RNA bacteriophages in vivo, quantification of AlkB-mediated repair of endogenous RNA and DNA in vivo, overview Escherichia coli