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Literature summary extracted from

  • Liu, S.; Liang, Q.M.; Huang, Y.J.; Yuan, X.; Zhou, W.W.; Qiao, F.; Cheng, J.; Gurr, G.M.; Zhu, Z.R.
    Cloning, functional characterization, and expression profiles of NADPH-cytochrome P450 reductase gene from the Asiatic rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) (2013), Comp. Biochem. Physiol. B, 166, 225-231.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 gene CsCPR, real-time quantitative PCR expression analysis and phylogenetic tree, heterologous expression of N-terminally truncated and His6-tagged protein in Escherichia coli Rosetta (DE3) cells Chilo suppressalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 additional information
-
 additional information CsCPR displays Michaelis-Menten behavior with respect to both cytochrome c and NADPH Chilo suppressalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 membrane bound, the N-terminal membrane anchor is functionally dispensable Chilo suppressalis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 76000
-
 x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation Chilo suppressalis
1.6.2.4 77600
-
 x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation Chilo suppressalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 NADPH + H+ + cytochrome c Chilo suppressalis
-
 NADP+ + reduced cytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Chilo suppressalis R4UB73 gene CsCPR
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 recombinant N-terminally truncated and His6-tagged protein from Escherichia coli Rosetta (DE3) cells to over 90% purity Chilo suppressalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 blood vessel dorsal Chilo suppressalis
-
1.6.2.4 fat body
-
 Chilo suppressalis
-
1.6.2.4 head
-
 Chilo suppressalis
-
1.6.2.4 integument
-
 Chilo suppressalis
-
1.6.2.4 leg
-
 Chilo suppressalis
-
1.6.2.4 malpighian tubule
-
 Chilo suppressalis
-
1.6.2.4 midgut
-
 Chilo suppressalis
-
1.6.2.4 additional information tissue- and developmental stage-dependent expression analysis of CsCPR mRNA by real-time quantitative PCR. The enzyme is exxpressed in the digestive, metabolic, and olfactory organs of the larvae and adults, overview Chilo suppressalis
-
1.6.2.4 nerve
-
 Chilo suppressalis
-
1.6.2.4 thorax
-
 Chilo suppressalis
-
1.6.2.4 trachea
-
 Chilo suppressalis
-
1.6.2.4 wing
-
 Chilo suppressalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 NADPH + H+ + cytochrome c
-
 Chilo suppressalis NADP+ + reduced cytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 76000, recombinant N-terminally truncated and His6-tagged protein, SDS-PAGE, x * 77600, about, sequence calculation Chilo suppressalis

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Chilo suppressalis
1.6.2.4 NADPH-cytochrome P450 reductase
-
 Chilo suppressalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.2.4 25
-
 assay at Chilo suppressalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.5
-
 assay at Chilo suppressalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Chilo suppressalis
1.6.2.4 FMN
-
 Chilo suppressalis
1.6.2.4 NADPH
-
 Chilo suppressalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.2.4 Chilo suppressalis sequence calculation
-
 5.6

General Information

EC Number General Information Comment Organism
1.6.2.4 physiological function NADPH-cytochrome P450 reductase is one of the most important components of the cytochrome P450 enzyme system. It catalyzes electron transfer from NADPH to all known P450s, thus plays central roles not only in the metabolism of exogenous xenobiotics but also in the regulation of endogenous hormones in insects Chilo suppressalis