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Literature summary extracted from
- Probing the dimerization interface of Leishmania infantum trypanothione reductase with site-directed mutagenesis and short peptides (2013), ChemBioChem, 14, 1212-1217.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.12 | expressed in Escherichia coli BL21(DE3) cells | Leishmania infantum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | E436A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Leishmania infantum |
| 1.8.1.12 | Q439A | the mutant shows slightly increased catalytic efficiency compared to the wild type enzyme | Leishmania infantum |
| 1.8.1.12 | W81A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Leishmania infantum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | Ac-PAIIQSVGISNLKNL-NH2 | about 30% residual activity at 0.025 mM | Leishmania infantum |  |
| 1.8.1.12 | Ac-PEIIQSVGISMKM-NH2 | - |
Leishmania infantum | |
| 1.8.1.12 | Ac-PEIIQSVGISNLKNL-NH2 | - |
Leishmania infantum | |
| 1.8.1.12 | Ac-PKAIQSVGISNLKNL-NH2 | about 18% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIASVGISNLKNL-NH2 | about 65% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQAVGISNLKNL-NH2 | about 10% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSAGISNLKNL-NH2 | about 19% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVAISNLKNL-NH2 | about 20% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGASNLKNL-NH2 | about 22% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGIANLKNL-NH2 | about 15% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGISAKNL-NH2 | about 30% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGISMKM-NH2 | the enzyme is almost completely inactivated after 50 min (3000 s) in the presence of 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGISNLANL-NH2 | about 31% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGISNLKA-NH2 | about 31% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | Ac-PKIIQSVGISNLKNL-NH2 | about 20% residual activity at 0.025 mM | Leishmania infantum | |
| 1.8.1.12 | additional information | not inhibited by Ac-KMGISSMVQPKII-NH2 and Ac-KIIPKVQGMSIMS-NH2 | Leishmania infantum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.0206 | - |
trypanothione disulfide | wild type enzyme, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 0.0253 | - |
trypanothione disulfide | mutant enzyme Q439A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 0.0493 | - |
trypanothione disulfide | mutant enzyme W81A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 0.1922 | - |
trypanothione disulfide | mutant enzyme E436A, pH and temperature not specified in the publication | Leishmania infantum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 60000 | - |
x * 60000, SDS-PAGE | Leishmania infantum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.12 | trypanothione disulfide + NADPH + H+ | Leishmania infantum | - |
trypanothione + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.12 | Leishmania infantum | A4HSF7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.12 | Ni-NTA column chromatography | Leishmania infantum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.12 | trypanothione disulfide + NADPH + H+ | - |
Leishmania infantum | trypanothione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | ? | x * 60000, SDS-PAGE | Leishmania infantum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | trypanothione reductase | - |
Leishmania infantum |
| 1.8.1.12 | TryR | - |
Leishmania infantum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.8 | - |
trypanothione disulfide | mutant enzyme E436A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 16.6 | - |
trypanothione disulfide | mutant enzyme W81A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 30.2 | - |
trypanothione disulfide | wild type enzyme, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 40.8 | - |
trypanothione disulfide | mutant enzyme Q439A, pH and temperature not specified in the publication | Leishmania infantum | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | NADPH | - |
Leishmania infantum | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.0157 | - |
pH and temperature not specified in the publication | Leishmania infantum | Ac-PKIIQSVGISNLKNL-NH2 | |
| 1.8.1.12 | 0.0179 | - |
pH and temperature not specified in the publication | Leishmania infantum | Ac-PKIIQSVGISMKM-NH2 | |
| 1.8.1.12 | 0.0336 | - |
pH and temperature not specified in the publication | Leishmania infantum | Ac-PEIIQSVGISNLKNL-NH2 | |
| 1.8.1.12 | 0.0408 | - |
pH and temperature not specified in the publication | Leishmania infantum | Ac-PEIIQSVGISMKM-NH2 | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 4 | - |
trypanothione disulfide | mutant enzyme E436A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 300 | - |
trypanothione disulfide | mutant enzyme W81A, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 1500 | - |
trypanothione disulfide | wild type enzyme, pH and temperature not specified in the publication | Leishmania infantum | |
| 1.8.1.12 | 1600 | - |
trypanothione disulfide | mutant enzyme Q439A, pH and temperature not specified in the publication | Leishmania infantum | |
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