Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Winter, R.T.; van den Berg, T.E.; Colpa, D.I.; van Bloois, E.; Fraaije, M.W.
    Functionalization of oxidases with peroxidase activity creates oxiperoxidases: a new breed of hybrid enzyme capable of cascade chemistry (2012), ChemBioChem, 13, 252-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.41 functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.41 additional information construction of an AldO mutant synthetic bifunctional enzyme, the enzyme from Streptomyces coelicolor A3(2) is endowed with an extra catalytic functionality by fusing it to a microperoxidase. The mutant is functional and a both fully covalently flavinylated and heminylated: an oxiperoxidase. Replacement of portions of the wild-type AldO sequence with the bacterial cytochrome c CXXCH heme-binding motif, domain structure, overview Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.41 0.29 0.35 xylitol recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.41 50000
-
 x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.41 xylitol + O2 Streptomyces coelicolor
-
 xylose + H2O2
-
 ?
1.1.3.41 xylitol + O2 Streptomyces coelicolor A3(2)
-
 xylose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.41 Streptomyces coelicolor Q9ZBU1
-
-
1.1.3.41 Streptomyces coelicolor A3(2) Q9ZBU1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.41 recombinant oxidase-peroxidase fusion mutant enzyme from Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.41 xylitol + O2
-
 Streptomyces coelicolor xylose + H2O2
-
 ?
1.1.3.41 xylitol + O2
-
 Streptomyces coelicolor A3(2) xylose + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.41 ? x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.3.41 AldO
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.41 9.1 12.2 xylitol recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.41 FAD
-
 Streptomyces coelicolor