Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, T.L.; Choroba, O.W.; Charles, E.H.; Sandercock, A.M.; Williams, D.H.; Spencer, J.B.
    Characterisation of a hydroxymandelate oxidase involved in the biosynthesis of two unusual amino acids occurring in the vancomycin group of antibiotics (2001), Chem. Commun. (Camb. ), 21, 1752-1753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.46 expression in Escherichia coli Amycolatopsis orientalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.46 39863
-
 x * 39863, electrospray ionization mass spectrometry Amycolatopsis orientalis
1.1.3.46 39864
-
 x * 39864, calculated from sequence Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.46 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 Amycolatopsis orientalis (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate i.e. (S)-4-hydroxymandelate. The enzyme is involed in the biosynthesis of (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine, two non-proteinogenic amino acids occurring in the vancomycin group of antibiotics 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 2-(4-hydroxyphenyl)-2-oxoacetate i.e. 4-hydroxyphenylglyoxylate ?
1.1.3.46 (S)-3,5-dihydroxymandelate + O2 Amycolatopsis orientalis the enzyme is involed in the biosynthesis of (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine, two non-proteinogenic amino acids occurring in the vancomycin group of antibiotics 2-(3,5-dihydroxyphenyl)-2-oxoacetate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.46 Amycolatopsis orientalis G4V4S8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.46
-
 Amycolatopsis orientalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.46 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate i.e. (S)-4-hydroxymandelate. The enzyme is involed in the biosynthesis of (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine, two non-proteinogenic amino acids occurring in the vancomycin group of antibiotics Amycolatopsis orientalis 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 2-(4-hydroxyphenyl)-2-oxoacetate i.e. 4-hydroxyphenylglyoxylate ?
1.1.3.46 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate i.e. (S)-4-hydroxymandelate Amycolatopsis orientalis 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 2-(4-hydroxyphenyl)-2-oxoacetate i.e. 4-hydroxyphenylglyoxylate ?
1.1.3.46 (S)-3,5-dihydroxymandelate + O2
-
 Amycolatopsis orientalis 2-(3,5-dihydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-3,5-dihydroxymandelate + O2 the enzyme is involed in the biosynthesis of (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine, two non-proteinogenic amino acids occurring in the vancomycin group of antibiotics Amycolatopsis orientalis 2-(3,5-dihydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-mandelate + O2 no activity with (R)-mandelate Amycolatopsis orientalis 2-phenyl-2-oxoacetate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.46 ? x * 39863, electrospray ionization mass spectrometry Amycolatopsis orientalis
1.1.3.46 ? x * 39864, calculated from sequence Amycolatopsis orientalis

Synonyms

EC Number Synonyms Comment Organism
1.1.3.46 4HmO
-
 Amycolatopsis orientalis
1.1.3.46 HmO
-
 Amycolatopsis orientalis
1.1.3.46 hydroxymandelate oxidase
-
 Amycolatopsis orientalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.46 25
-
 assay at Amycolatopsis orientalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.46 7.6
-
 assay at Amycolatopsis orientalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.46 FMN flavoprotein Amycolatopsis orientalis

General Information

EC Number General Information Comment Organism
1.1.3.46 physiological function the enzyme is involed in the biosynthesis of L-(4-hydroxyphenyl)glycine and L-(3,5-dihydroxyphenyl)glycine, two non-proteinogenic amino acids occurring in the vancomycin group of antibiotics Amycolatopsis orientalis