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Literature summary extracted from

  • Celik, H.; Ko?ar, M.
    Inhibitory effects of dietary flavonoids on purified hepatic NADH-cytochrome b5 reductase: structure-activity relationships (2012), Chem. Biol. Interact., 197, 103-109.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.6.2.2 drug development flavonoids, regarding b5 reductase inhibition, indicate a potential for significant flavonoid–drug and/or flavonoid–xenobiotic interactions which may have important therapeutic and toxicological outcomes for certain drugs and/or xenobiotics. Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 (+)-catechin
-
 Bos taurus
1.6.2.2 (-)-epicatechin
-
 Bos taurus
1.6.2.2 apigenin
-
 Bos taurus
1.6.2.2 luteolin
-
 Bos taurus
1.6.2.2 luteolin-7-O-glucoside
-
 Bos taurus
1.6.2.2 additional information inhibitory potencies of flavonoids on the enzyme, structure-activity relationship, overview. No inhibition by naringenin, naringin, and chrysin. Flavonoids containing two hydroxyl groups in ring B and a carbonyl group at C-4 in combination with a double bond between C-2 and C-3 produced a much stronger inhibition, whereas substitution of a hydroxyl group at C-3 is associated with a less inhibitory effect Bos taurus
1.6.2.2 morin
-
 Bos taurus
1.6.2.2 myricetin
-
 Bos taurus
1.6.2.2 quercetin
-
 Bos taurus
1.6.2.2 quercitrin
-
 Bos taurus
1.6.2.2 rutin
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 microsome
-
 Bos taurus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 native enzyme from liver microsomes by anion exchange chromatography and affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Bos taurus 2 ferrocyanide + NAD+ + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.2 cytochrome b5 reductase
-
 Bos taurus
1.6.2.2 NADH-cytochrome b5 reductase
-
 Bos taurus
1.6.2.2 NADH-ferricyanide reductase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.2.2 22
-
 assay at room temperature Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 7.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.2 additional information
-
 additional information inhibition kinetics Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.6.2.2 0.00011
-
 pH 7.5, 22°C Bos taurus luteolin
1.6.2.2 0.00081
-
 pH 7.5, 22°C Bos taurus morin
1.6.2.2 0.0011
-
 pH 7.5, 22°C Bos taurus quercetin
1.6.2.2 0.0012
-
 pH 7.5, 22°C Bos taurus quercitrin
1.6.2.2 0.0015
-
 pH 7.5, 22°C Bos taurus myricetin
1.6.2.2 0.0024
-
 pH 7.5, 22°C Bos taurus luteolin-7-O-glucoside
1.6.2.2 0.0032
-
 pH 7.5, 22°C Bos taurus (-)-epicatechin
1.6.2.2 0.0045
-
 pH 7.5, 22°C Bos taurus (+)-catechin
1.6.2.2 0.036
-
 pH 7.5, 22°C Bos taurus apigenin
1.6.2.2 0.057
-
 pH 7.5, 22°C Bos taurus rutin

General Information

EC Number General Information Comment Organism
1.6.2.2 additional information structure-activity relationship, overview Bos taurus