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Literature summary extracted from

  • Amenabar, M.J.; Blamey, J.M.
    Purification and characterization of a thermostable glutamate dehydrogenase from a thermophilic bacterium isolated from a sterilization drying oven (2012), BMB Rep., 45, 91-95.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.3 0.1
-
 NADP+ pH 8.0, 70°C uncultured bacterium
1.4.1.3 0.3
-
 L-glutamate pH 8.0, 70°C uncultured bacterium
1.4.1.3 0.4
-
 NAD+ pH 8.0, 70°C uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 40500
-
 6 * 40500, SDS-PAGE uncultured bacterium
1.4.1.3 250500
-
 gel filtration uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD+ uncultured bacterium
-
 2-oxoglutarate + NH3 + NADH + H+
-
 ir
1.4.1.3 L-glutamate + H2O + NADP+ uncultured bacterium
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 uncultured bacterium
-
 GWE1, a Gram-positive microaerophilic microorganism isolated from from the interior of a sterilization drying oven
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3 native enzyme 22.7fold by anion exchange chromatography and gel filtration to homogeneity uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 79.4
-
 purified enzyme, pH 8.0, 70°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD+
-
 uncultured bacterium 2-oxoglutarate + NH3 + NADH + H+
-
 ir
1.4.1.3 L-glutamate + H2O + NADP+
-
 uncultured bacterium 2-oxoglutarate + NH3 + NADPH + H+
-
 ir
1.4.1.3 additional information no activity in the amination reaction direction with NADH or NADPH, 2-oxoglutarate and ammonia uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 40500, SDS-PAGE uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 70
-
 glutamate oxidation uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.3 50 100 activity range, profile overview. Instability of the coenzyme at temperatures above 95°C uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 100
-
 purified enzyme, 70% activity remaining after over 5 h uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 8
-
 glutamate oxidation uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.3 7.9 9 activity range, profile overview uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.3 additional information the enzyme can use both NAD+ and NADP+ as electron acceptors, displaying more affinity for NADP+ than for NAD+. No activity with NADH or NADPH uncultured bacterium
1.4.1.3 NAD+
-
 uncultured bacterium
1.4.1.3 NADP+
-
 uncultured bacterium