BRENDA - Enzyme Database show

Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil

Lu, Y.; Yu, Y.; Zhou, R.; Sun, W.; Dai, C.; Wan, P.; Zhang, L.; Hao, D.; Ren, H.; Biotechnol. Lett. 33, 1159-1167 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.20
expression in Escherichia coli
Soil bacterium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.20
Cu2+
0.1 mM, complete loss of activity
Soil bacterium
1.14.13.20
Hg2+
0.1 mM, complete loss of activity
Soil bacterium
1.14.13.20
Zn2+
0.1 mM, complete loss of activity
Soil bacterium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.005
-
2,4-Dichlorophenol
pH 7.5, 25°C
Soil bacterium
1.14.13.20
0.006
-
NADPH
pH 7.5, 25°C
Soil bacterium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.20
Ca2+
0.1 mM, 130% of initial activity
Soil bacterium
1.14.13.20
Co2+
0.1 mM, 110% of initial activity
Soil bacterium
1.14.13.20
Mn2+
0.1 mM, 121% of initial activity
Soil bacterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.20
Soil bacterium
-
metagenome constructed from polychlorinated biphenyl-contaminated soil
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + O2
113% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3,4-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
-
724636
Soil bacterium
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
103% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3,6-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
178% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2-bromo-4-chlorophenol + NADPH + O2
82% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3-bromo-5-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
129% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
3-chlorophenol + NADPH + O2
230% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
4-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
4-bromo-2-chlorophenol + NADPH + O2
86% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
5-bromo-3-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
95% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
5-chloro-3-methylcatechol + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
25
-
-
Soil bacterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.5
-
-
Soil bacterium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.20
expression in Escherichia coli
Soil bacterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.20
Cu2+
0.1 mM, complete loss of activity
Soil bacterium
1.14.13.20
Hg2+
0.1 mM, complete loss of activity
Soil bacterium
1.14.13.20
Zn2+
0.1 mM, complete loss of activity
Soil bacterium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.005
-
2,4-Dichlorophenol
pH 7.5, 25°C
Soil bacterium
1.14.13.20
0.006
-
NADPH
pH 7.5, 25°C
Soil bacterium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.20
Ca2+
0.1 mM, 130% of initial activity
Soil bacterium
1.14.13.20
Co2+
0.1 mM, 110% of initial activity
Soil bacterium
1.14.13.20
Mn2+
0.1 mM, 121% of initial activity
Soil bacterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + O2
113% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3,4-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + H+ + O2
-
724636
Soil bacterium
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
103% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3,6-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
178% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
? + NADP+ + H2O
-
-
-
?
1.14.13.20
2-bromo-4-chlorophenol + NADPH + O2
82% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3-bromo-5-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
129% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
3-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
3-chlorophenol + NADPH + O2
230% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
4-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
4-bromo-2-chlorophenol + NADPH + O2
86% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
5-bromo-3-chlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
95% of the activity with 2,4-dichlorophenol
724636
Soil bacterium
5-chloro-3-methylcatechol + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
25
-
-
Soil bacterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.5
-
-
Soil bacterium