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Literature summary extracted from

  • Lu, Y.; Yu, Y.; Zhou, R.; Sun, W.; Dai, C.; Wan, P.; Zhang, L.; Hao, D.; Ren, H.
    Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil (2011), Biotechnol. Lett., 33, 1159-1167.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.20 expression in Escherichia coli Soil bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.20 Cu2+ 0.1 mM, complete loss of activity Soil bacterium
1.14.13.20 Hg2+ 0.1 mM, complete loss of activity Soil bacterium
1.14.13.20 Zn2+ 0.1 mM, complete loss of activity Soil bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.20 0.005
-
 2,4-Dichlorophenol pH 7.5, 25°C Soil bacterium
1.14.13.20 0.006
-
 NADPH pH 7.5, 25°C Soil bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.20 Ca2+ 0.1 mM, 130% of initial activity Soil bacterium
1.14.13.20 Co2+ 0.1 mM, 110% of initial activity Soil bacterium
1.14.13.20 Mn2+ 0.1 mM, 121% of initial activity Soil bacterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.20 Soil bacterium
-
 metagenome constructed from polychlorinated biphenyl-contaminated soil
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.20 2,3-dichlorophenol + NADPH + O2 113% of the activity with 2,4-dichlorophenol Soil bacterium 3,4-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,4-dichlorophenol + NADPH + H+ + O2
-
 Soil bacterium 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,5-dichlorophenol + NADPH + O2 103% of the activity with 2,4-dichlorophenol Soil bacterium 3,6-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,5-dichlorophenol + NADPH + O2 178% of the activity with 2,4-dichlorophenol Soil bacterium ? + NADP+ + H2O
-
 ?
1.14.13.20 2-bromo-4-chlorophenol + NADPH + O2 82% of the activity with 2,4-dichlorophenol Soil bacterium 3-bromo-5-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2-chlorophenol + NADPH + O2 129% of the activity with 2,4-dichlorophenol Soil bacterium 3-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 3-chlorophenol + NADPH + O2 230% of the activity with 2,4-dichlorophenol Soil bacterium 4-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 4-bromo-2-chlorophenol + NADPH + O2 86% of the activity with 2,4-dichlorophenol Soil bacterium 5-bromo-3-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 4-chloro-2-methylphenol + NADPH + O2 95% of the activity with 2,4-dichlorophenol Soil bacterium 5-chloro-3-methylcatechol + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.20 TfdB-JLU
-
 Soil bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.20 25
-
-
 Soil bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.20 7.5
-
-
 Soil bacterium