Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cui, Z.; Zhang, Y.; Inoue, H.; Yogo, S.; Hirasawa, E.
    Purification and molecular analysis of a monoamine oxidase isolated from Narcissus tazetta (2013), Biosci. Biotechnol. Biochem., 77, 1728-1733.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 2,2'-bipyridyl 50% inhibition at 1 mM Narcissus tazetta
1.4.3.4 2-Bromoethylamine complete inhibition at 0.01 mM Narcissus tazetta
1.4.3.4 additional information hardly inhibited by 1 mM o-phenanthroline (4%), pargyline (2%) and clorgyline (1%) Narcissus tazetta
1.4.3.4 Semicarbazide complete inhibition at 0.01 mM Narcissus tazetta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 0.059
-
n-Propylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 0.11
-
methylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 0.11
-
n-butylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 0.13
-
ethylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 0.41
-
benzylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 0.5
-
n-Pentylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 1.4
-
2-Phenylethylamine at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 2
-
ethanolamine at pH 7.0 and 37°C Narcissus tazetta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.4 Cu2+ the enzyme contains 2.44 mol of copper atoms per mole of enzyme Narcissus tazetta
1.4.3.4 Mn2+ 133% activity at 0.1 mM Mn2+ Narcissus tazetta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.4 65000
-
1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta
1.4.3.4 75000
-
1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta
1.4.3.4 135000
-
gel filtration Narcissus tazetta

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Narcissus tazetta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.4 ammonium sulfate precipitation, Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, and hydroxyapatite column chromatography Narcissus tazetta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.4 shoot
-
Narcissus tazetta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.4 0.0032
-
crude extract, at pH 7.0 and 37°C Narcissus tazetta
1.4.3.4 1.365
-
1544fold purified enzyme, at pH 7.0 and 37°C Narcissus tazetta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 2-phenylethylamine + H2O + O2 9% activity compared to n-propylamine Narcissus tazetta 2-phenylethanal + NH3 + H2O2
-
?
1.4.3.4 benzylamine + H2O + O2 74% activity compared to n-propylamine Narcissus tazetta benzaldehyde + NH3 + H2O2
-
?
1.4.3.4 ethanolamine + H2O + O2 50% activity compared to n-propylamine Narcissus tazetta glycolaldehyde + NH3 + H2O2
-
?
1.4.3.4 ethylamine + H2O + O2 84% activity compared to n-propylamine Narcissus tazetta ethanal + NH3 + H2O2
-
?
1.4.3.4 hexylamine + H2O + O2 7% activity compared to n-propylamine Narcissus tazetta hexanal + NH3 + H2O2
-
?
1.4.3.4 methylamine + H2O + O2 34% activity compared to n-propylamine Narcissus tazetta formaldehyde + NH3 + H2O2
-
?
1.4.3.4 additional information no activity with tryptamine, serotonin, dopamine, tyramine, histamine, putrescine, cadaverine, diaminodecane, spermidine, and spermine Narcissus tazetta ?
-
?
1.4.3.4 n-butylamine + H2O + O2 98% activity compared to n-propylamine Narcissus tazetta butyraldehyde + NH3 + H2O2
-
?
1.4.3.4 n-pentylamine + H2O + O2 56% activity compared to n-propylamine Narcissus tazetta pentaldehyde + NH3 + H2O2
-
?
1.4.3.4 n-propylamine + H2O + O2 100% activity Narcissus tazetta propionaldehyde + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.4 heterodimer 1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO
-
Narcissus tazetta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.4 50 80 the purified enzyme retains its activity at temperatures up to 70°C but loses activity quickly after pre-incubation at temperatures of 75°C (less than 50%) and higher Narcissus tazetta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.4 7
-
-
Narcissus tazetta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.4 6 8
-
Narcissus tazetta

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.4 FAD
-
Narcissus tazetta

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.4.3.4 1
-
at pH 7.0 and 37°C Narcissus tazetta 2,2'-bipyridyl