Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maruta, T.; Ichikawa, Y.; Mieda, T.; Takeda, T.; Tamoi, M.; Yabuta, Y.; Ishikawa, T.; Shigeoka, S.
    The contribution of Arabidopsis homologs of L-gulono-1,4-lactone oxidase to the biosynthesis of ascorbic acid (2010), Biosci. Biotechnol. Biochem., 74, 1494-1497.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.8 gene AtGulLO2, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana
1.1.3.8 gene AtGulLO3, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana
1.1.3.8 gene AtGulLO5, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana
1.1.3.37 gene AtGulLO2, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana
1.1.3.37 gene AtGulLO3, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana
1.1.3.37 gene AtGulLO5, transgenic expression of the isozyme in Nicotiana tabacum BY-2 cells, the overexpression does not affect the cells. Expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)pLysS is not successful Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Arabidopsis thaliana
-
 L-ascorbate + H2O2
-
 ?
1.1.3.8 additional information Arabidopsis thaliana the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37 ?
-
 ?
1.1.3.37 additional information Arabidopsis thaliana the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producing L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 Arabidopsis thaliana O81030 AtGulLO5; isozyme AtGulLO5
-
1.1.3.8 Arabidopsis thaliana Q6NQ66 AtGulLO2; isozyme AtGulLO2
-
1.1.3.8 Arabidopsis thaliana Q9LYD8 AtGulLO3; isozyme AtGulLO3
-
1.1.3.37 Arabidopsis thaliana O81030 AtGulLO5; isozyme AtGulLO5
-
1.1.3.37 Arabidopsis thaliana Q6NQ66 AtGulLO2; isozyme AtGulLO2
-
1.1.3.37 Arabidopsis thaliana Q9LYD8 AtGulLO3; isozyme AtGulLO3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Arabidopsis thaliana L-ascorbate + H2O2
-
 ?
1.1.3.8 additional information the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37 Arabidopsis thaliana ?
-
 ?
1.1.3.37 additional information the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producing L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.8 AtGulLO2
-
 Arabidopsis thaliana
1.1.3.8 AtGulLO3
-
 Arabidopsis thaliana
1.1.3.8 AtGulLO5
-
 Arabidopsis thaliana
1.1.3.8 L-GulL oxidase
-
 Arabidopsis thaliana
1.1.3.8 L-gulono-1,4-lactone oxidase
-
 Arabidopsis thaliana
1.1.3.37 ALO
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.8 25
-
 assay at Arabidopsis thaliana
1.1.3.37 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.8 6
-
 assay at Arabidopsis thaliana
1.1.3.37 6
-
 assay at Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.1.3.8 Arabidopsis thaliana L-gulono-1,4-lactone induces the enzyme, overview up
1.1.3.37 Arabidopsis thaliana L-gulono-1,4-lactone induces the enzyme, overview up