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Literature summary extracted from

  • Jamil, F.; Rashid, N.; Gardner, Q.; Akhtar, M.
    Gene cloning and characterization of glycine oxidase from newly isolated Bacillus subtilis strain R5 (2011), Biologia, 66, 1-7.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3)CodonPlus-RIL cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.19 acetone 22% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
1.4.3.19 acetonitrile complete inhibition at 30% (v/v) Bacillus subtilis
1.4.3.19 Co2+ there is a sharp decrease in activity when 1 mM Co2+ is added to the reaction assay Bacillus subtilis
1.4.3.19 diethylether 73% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
1.4.3.19 ethyl acetate 19% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
1.4.3.19 methanol 54% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
1.4.3.19 N,N-Dimethyl formamide 17% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
1.4.3.19 Zn2+ there is a sharp decrease in activity when 1 mM Zn2+ is added to the reaction assay Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.37
-
 glycine at pH 8.0 and 50°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.19 additional information no enhancement of enzyme activity in the presence of metal cations Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.19 40000
-
 4 * 40000, SDS-PAGE Bacillus subtilis
1.4.3.19 40760
-
 4 * 40760, MALDI-TOF mass spectrometry Bacillus subtilis
1.4.3.19 40761
-
 4 * 40761, calculated from amino acid sequence Bacillus subtilis
1.4.3.19 160000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2 Bacillus subtilis
-
 pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-alanine + H2O + O2 Bacillus subtilis R5
-
 pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2 Bacillus subtilis
-
 glyoxylate + NH3 + H2O2
-
 r
1.4.3.19 glycine + H2O + O2 Bacillus subtilis R5
-
 glyoxylate + NH3 + H2O2
-
 r
1.4.3.19 additional information Bacillus subtilis no activity with L-alanine ?
-
 ?
1.4.3.19 additional information Bacillus subtilis R5 no activity with L-alanine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus subtilis D7UTZ8
-
-
1.4.3.19 Bacillus subtilis R5 D7UTZ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 ammonium sulfate precipitation, Resource Q column chromatography and Resource ISO column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.19 14
-
 cell free extract, when glycine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis
1.4.3.19 960
-
 after 68.6fold purification, when glycine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis
1.4.3.19 1000
-
 after 68.6fold purification, when D-alanine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 D-alanine + H2O + O2
-
 Bacillus subtilis pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-alanine + H2O + O2
-
 Bacillus subtilis R5 pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis glyoxylate + NH3 + H2O2
-
 r
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis R5 glyoxylate + NH3 + H2O2
-
 r
1.4.3.19 additional information no activity with L-alanine Bacillus subtilis ?
-
 ?
1.4.3.19 additional information no activity with L-alanine Bacillus subtilis R5 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 homotetramer 4 * 40000, SDS-PAGE Bacillus subtilis
1.4.3.19 homotetramer 4 * 40760, MALDI-TOF mass spectrometry Bacillus subtilis
1.4.3.19 homotetramer 4 * 40761, calculated from amino acid sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 glycine:oxygen oxidoreductase (deaminating)
-
 Bacillus subtilis
1.4.3.19 GoxR
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.19 50
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.19 40 55 the enzyme exhibits almost 80% of its activity at temperatures 40 and 55°C. The activity sharply decreases above 55°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.19 7 9 about 75% activity at pH 7.0, more than 50% activity is retained between pH 7.5 and 8.5, about 60% activity at pH 9.0. The enzyme activity decreases sharply below pH 7.0 and above pH 9.0. There is a negligible amount of activity at pH below 5.0 and above 10.0 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD
-
 Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.19 Bacillus subtilis calculated from amino acid sequence
-
 5.8