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Literature summary extracted from

  • Raimo, G.; Masullo, M.; Scarano, G.; Bocchini, V.
    The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols (1996), Biochimie, 78, 832-837.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.3 1-propanol stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol Saccharolobus solfataricus
3.6.5.3 2-propanol stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol Saccharolobus solfataricus
3.6.5.3 ethanol stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol Saccharolobus solfataricus
3.6.5.3 ethylene glycoI 60%, 300fold stimulation Saccharolobus solfataricus
3.6.5.3 glycerol stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol Saccharolobus solfataricus
3.6.5.3 methanol stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.5.3 0.0009
-
 GTP pH 7.8, 50°C Saccharolobus solfataricus
3.6.5.3 0.001
-
 GTP pH 7.8, 75°C Saccharolobus solfataricus
3.6.5.3 0.0012
-
 GTP pH 7.8, 60°C Saccharolobus solfataricus
3.6.5.3 0.0013
-
 GTP pH 7.8, 80°C Saccharolobus solfataricus
3.6.5.3 0.0014
-
 GTP pH 7.8, 70°C Saccharolobus solfataricus
3.6.5.3 0.0053
-
 GTP pH 7.8, 87°C Saccharolobus solfataricus
3.6.5.3 0.02
-
 GTP pH 7.8, 91°C Saccharolobus solfataricus
3.6.5.3 0.0532
-
 GTP pH 7.8, 95°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.3 BaCl2 10 mM, about 8fold stimulation Saccharolobus solfataricus
3.6.5.3 CaCl2 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness Saccharolobus solfataricus
3.6.5.3 CoCl2 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness Saccharolobus solfataricus
3.6.5.3 MgCl2 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness Saccharolobus solfataricus
3.6.5.3 MnCl2 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness Saccharolobus solfataricus
3.6.5.3 additional information GTPase stimulated by ethylene glycol and BaCl2 does not require the presence of univalent cations. Li+, Na+, K+ or NH4+ added singularly up to 1 M concentration, do not produce any significant stimulation of SsEF-2 GTPase either in the absence or in the presence of ethylene glycol. They reduce the stimulation of SsEF-2 GTPase by ethylene glycol plus BaCl2 or SrCl2 Saccharolobus solfataricus
3.6.5.3 SrCl2 10 mM, highest stimulation by BaCl2 (8fold), followed by SrCl2, MgCl2, MnCl2, CaCl2 and CoCl2 in a decreasing order of effectiveness Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Saccharolobus solfataricus
-
-
-
3.6.5.3 Saccharolobus solfataricus ATCC 49255
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O
-
 Saccharolobus solfataricus ATCC 49255 GDP + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.5.3 60
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.5.3 0.023
-
 GTP pH 7.8, 50°C Saccharolobus solfataricus
3.6.5.3 0.038
-
 GTP pH 7.8, 60°C Saccharolobus solfataricus
3.6.5.3 0.118
-
 GTP pH 7.8, 70°C Saccharolobus solfataricus
3.6.5.3 0.152
-
 GTP pH 7.8, 75°C Saccharolobus solfataricus
3.6.5.3 0.23
-
 GTP pH 7.8, 80°C Saccharolobus solfataricus
3.6.5.3 0.512
-
 GTP pH 7.8, 87°C Saccharolobus solfataricus
3.6.5.3 0.558
-
 GTP pH 7.8, 95°C Saccharolobus solfataricus
3.6.5.3 0.89
-
 GTP pH 7.8, 91°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.5.3 11
-
 GTP pH 7.8, 95°C Saccharolobus solfataricus
3.6.5.3 26
-
 GTP pH 7.8, 50°C Saccharolobus solfataricus
3.6.5.3 32
-
 GTP pH 7.8, 60°C Saccharolobus solfataricus
3.6.5.3 45
-
 GTP pH 7.8, 91°C Saccharolobus solfataricus
3.6.5.3 84
-
 GTP pH 7.8, 70°C Saccharolobus solfataricus
3.6.5.3 97
-
 GTP pH 7.8, 87°C Saccharolobus solfataricus
3.6.5.3 152
-
 GTP pH 7.8, 75°C Saccharolobus solfataricus
3.6.5.3 177
-
 GTP pH 7.8, 80°C Saccharolobus solfataricus