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Literature summary extracted from

  • Gomes, C.M.; Lemos, R.S.; Teixeira, M.; Kletzin, A.; Huber, H.; Stetter, K.O.; Schäfer, G.; Anemüller, S.
    The unusual iron sulfur composition of the Acidianus ambivalens succinate dehydrogenase complex (1999), Biochim. Biophys. Acta, 1411, 134-141.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.1
-
 Acidianus ambivalens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.06
-
 fumarate pH 6.5, 70°C, detergent treated membranes from aerobic growth Acidianus ambivalens
1.3.5.1 0.06
-
 fumarate pH 6.5, 70°C, determined for activity in membranes from aerobic growth Acidianus ambivalens
1.3.5.1 0.08
-
 fumarate pH 6.5, 70°C, detergent treated membranes from anaerobic growth Acidianus ambivalens
1.3.5.1 0.3
-
 succinate pH 6.5, 70°C, detergent treated membranes from anaerobic growth Acidianus ambivalens
1.3.5.1 0.5
-
 succinate pH 6.5, 70°C, detergent treated membranes from aerobic growth Acidianus ambivalens
1.3.5.1 0.5
-
 succinate pH 6.5, 70°C, determined for activity in membranes from aerobic growth Acidianus ambivalens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 membrane
-
 Saccharolobus solfataricus 16020
-
1.3.5.1 membrane
-
 Acidianus ambivalens 16020
-
1.3.5.1 membrane
-
 Acidianus infernus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.1 Iron SdhB is the iron-sulfur subunit Acidianus ambivalens
1.3.5.1 Iron-sulfur cluster the enzyme has an unusual iron-sulfur cluster composition in respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present. The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed Saccharolobus solfataricus
1.3.5.1 Iron-sulfur cluster the enzyme has an unusual iron-sulfur cluster composition with respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present in membranes prepared from aerobically grown Acidianus ambivalens, nor in partially purified complex fractions. It is replaced by a second [4Fe-4S] center, by incorporation of an additional cysteine, at the cysteine cluster binding motif (CxxYxxCxxxC-->CxxCxxCxxxC). The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed Acidianus ambivalens
1.3.5.1 Iron-sulfur cluster the enzyme has an unusual iron-sulfur cluster composition with respect to that of the canonical succinate dehydrogenases. Center S3, the succinate responsive [3Fe-4S]1+/0 cluster of succinate dehydrogenases, is not present. The remaining centers, clusters S1 ([2Fe-2S]1+/2+) and S2 ([4Fe-4S]2+/1+), can be observed Acidianus infernus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 fumarate + reduced acceptor Acidianus ambivalens the obligate autotroph requires fumarate reductase activity if it performs CO2 fixation via a reductive citric acid cycle succinate + acceptor
-
 r
1.3.5.1 succinate + caldariellaquinone Acidianus ambivalens
-
 fumarate + caldariellaquinol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Acidianus ambivalens
-
-
-
1.3.5.1 Acidianus ambivalens O73937 and O73937 and O73939 and O73940 O73937: FAD-containing subunit SdhA, O73937: iron-sulfur subunit SdhB, O73939: subunit SdhC, O73940: subunit SdhD
-
1.3.5.1 Acidianus infernus
-
-
-
1.3.5.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1
-
 Acidianus ambivalens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.1 culture condition:aerobically-grown cell
-
 Acidianus ambivalens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + reduced acceptor the obligate autotroph requires fumarate reductase activity if it performs CO2 fixation via a reductive citric acid cycle Acidianus ambivalens succinate + acceptor
-
 r
1.3.5.1 fumarate + reduced benzyl viologen
-
 Acidianus ambivalens succinate + oxidized benzyl viologen
-
 r
1.3.5.1 succinate + caldariellaquinone
-
 Acidianus ambivalens fumarate + caldariellaquinol
-
 r
1.3.5.1 succinate + oxidized 2,6-dichlorophenolindophenol
-
 Acidianus ambivalens fumarate + reduced 2,6-dichlorophenolindophenol
-
 r
1.3.5.1 succinate + oxidized benzyl viologen
-
 Acidianus ambivalens fumarate + reduced benzyl viologen
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.1 70
-
 assay at Acidianus ambivalens
1.3.5.1 75 81 from 30°C to 75°C the activity increases 45fold, with an optimum temperature between 75°C and 81°C Acidianus ambivalens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.5.1 30 75 from 30°C to 75°C the activity increases 45fold, with an optimum temperature between 75°C and 81°C Acidianus ambivalens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 6.5
-
 assay at Acidianus ambivalens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 FAD SdhA is the FAD-containing subunit Acidianus ambivalens