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Literature summary extracted from

  • Hamon, V.; Dallet, S.; Legoy, M.D.
    The pressure-dependence of two beta-glucosidases with respect to their thermostability (1996), Biochim. Biophys. Acta, 1294, 195-203.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B26 4-nitrophenyl-beta-D-galactopyranoside at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate Saccharolobus solfataricus
3.2.1.B26 additional information this enzyme appears to be activated by pressure between atmospheric pressure and 2.5 kbar with a maximal activity at 1.25 kbar. However, this enzyme still displayed the best catalytic efficiency at atmospheric pressure because of a Km value drastically increased by pressure Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
3.2.1.B26 highly barostable enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B26 4-nitrophenyl-beta-D-galactopyranoside at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.085
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1 bar Saccharolobus solfataricus
3.2.1.B26 0.16
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 500 bar Saccharolobus solfataricus
3.2.1.B26 0.41
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 750 bar Saccharolobus solfataricus
3.2.1.B26 0.55
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1000 bar Saccharolobus solfataricus
3.2.1.B26 0.86
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1500 bar Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 100
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B26 80 120 80°C: about 40% of maximal activity, 110°C: 85% of maximal activity, 120°C: about 25% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 5
-
 after a storage greater than 40 h at atmospheric pressure, the residual activity decreases Saccharolobus solfataricus
3.2.1.B26 60
-
 maintains a half-life of 91 h at 60°C and 2.5 kbar Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 1764
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1500 bar Saccharolobus solfataricus
3.2.1.B26 3356
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1000 bar Saccharolobus solfataricus
3.2.1.B26 5811
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 750 bar Saccharolobus solfataricus
3.2.1.B26 11130
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 500 bar Saccharolobus solfataricus
3.2.1.B26 11910
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C, 1 bar Saccharolobus solfataricus