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Literature summary extracted from

  • Liu, J.; Murkin, A.S.
    Pre-steady-state kinetic analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Mycobacterium tuberculosis reveals partially rate-limiting product release by parallel pathways (2012), Biochemistry, 51, 5307-5319.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.267 drug development because the enzyme is absent in humans, DXR is a target for drug discovery Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 expression of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.267 additional information construction of an N-terminal His6-tagged enzyme variant comprising the first 389 of 413 residues, the mutant is more stable and kinetically similar to the wild-type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 NADP+ product inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 additional information
-
 additional information transient kinetics and kinetic mechanism, stopped-flow fluorescence measurements, overview. Kinetics of pre-steady-state reactions and steady-state kinetics, comparison of simulated and experimental values, burst kinetics model for pre-steady-state kinetics Mycobacterium tuberculosis
1.1.1.267 0.0098
-
 NADPH pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis
1.1.1.267 0.084
-
 2-C-methyl-D-erythritol 4-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 0.115
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 0.42
-
 NADP+ pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Mycobacterium tuberculosis
-
-
-
1.1.1.267 no activity in Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ random substrate binding and ordered release of NADP+ followed by partially rate-limiting release of 2-C-methyl-D-erythritol 4-phosphate, transient generation of MtDXR-NADPH-MEP ternary complex, kinetic analysis and modeling, overview Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ NADPH and 2-C-methyl-D-erythritol 4-phosphate bind to the free enzyme and the two bind together to generate a nonproductive ternary complex Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose-5-phosphate reductoisomerase
-
 Mycobacterium tuberculosis
1.1.1.267 DXP reductoisomerase
-
 Mycobacterium tuberculosis
1.1.1.267 DXR
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 25
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 0.95
-
 2-C-methyl-D-erythritol 4-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 0.95
-
 NADP+ pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis
1.1.1.267 5.25
-
 NADPH pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 5.25
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.267 5.7
-
 NADP+ pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis
1.1.1.267 11.3
-
 2-C-methyl-D-erythritol 4-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 46
-
 1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
1.1.1.267 540
-
 NADPH pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis