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Literature summary extracted from

  • Dassama, L.M.; Yosca, T.H.; Conner, D.A.; Lee, M.H.; Blanc, B.; Streit, B.R.; Green, M.T.; DuBois, J.L.; Krebs, C.; Bollinger, J.M.
    O2-evolving chlorite dismutase as a tool for studying O2-utilizing enzymes (2012), Biochemistry, 51, 1607-1616.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.11.49 molecular biology use of the heme enzyme for the rapid, in situ generation of O2 at concentrations far exceeding 2 mM in coupled enzyme reaction systems to study O consumption or O2 involving reaction steps. Catalytic concentrations of chlorite O2-lyase can be used to initiate the reaction of an O2-utilizing (metallo)enzyme by rapid mixing with the highly soluble, non-volatile ClO2, rather than with the sparingly soluble, gaseous O2, e.g. activation of the beta2 subunit of class Ic ribonucleotide reductase from Chlamydia trachomatis by mixing its MnII/FeII complex with ClO2- in the presence of chlorite O2-lyase, overview Dechloromonas aromatica

General Stability

EC Number General Stability Organism
1.13.11.49 the enzyme is capable of approximately 17000 turnovers per heme before undergoing irreversible inactivation due to oxidative damage to the heme Dechloromonas aromatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.49 additional information no product inhibition by O2 or chloride at millimolar concentrations Dechloromonas aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 additional information
-
 additional information kinetic mechanism, overview Dechloromonas aromatica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.49 Fe2+ heme enzyme Dechloromonas aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chlorite Dechloromonas aromatica the heme enzyme rapidly converts chlorite to chloride and O2, suggesting a simple approach to overcoming both the technical difficulties in the systematic variation of the O2 concentration and its modest solubility chloride + O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Dechloromonas aromatica
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.49 the enzyme is capable of approximately 17000 turnovers per heme before undergoing irreversible inactivation due to oxidative damage to the heme Dechloromonas aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Dechloromonas aromatica chloride + O2
-
 ?
1.13.11.49 chlorite the heme enzyme rapidly converts chlorite to chloride and O2, suggesting a simple approach to overcoming both the technical difficulties in the systematic variation of the O2 concentration and its modest solubility Dechloromonas aromatica chloride + O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.49 homopentamer
-
 Dechloromonas aromatica

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 chlorite dismutase
-
 Dechloromonas aromatica
1.13.11.49 CLD
-
 Dechloromonas aromatica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.49 200000
-
 Chlorite pH 5.2, 4°C Dechloromonas aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.49 heme
-
 Dechloromonas aromatica

General Information

EC Number General Information Comment Organism
1.13.11.49 additional information the enzyme is capable of approximately 17000 turnovers per heme before undergoing irreversible inactivation due to oxidative damage to the heme Dechloromonas aromatica
1.13.11.49 physiological function the enzyme detoxifies the chlorite that results from chlorate respiration in bacteria and would otherwise accumulate in the cell and kill the organism. The reaction must be fast in order to serve its biological function: the homopentameric enzyme from Dechloromonas aromatica is one of the fastest and most efficient chlorite O2-lyases yet studied. The enzyme can support the generation of tens-of-millimolar O2 on the millisecond timescale Dechloromonas aromatica