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Literature summary extracted from

  • Rajapaksha, M.; Thomas, J.L.; Streeter, M.; Prasad, M.; Whittal, R.M.; Bell, J.D.; Bose, H.S.
    Lipid-mediated unfolding of 3beta-hydroxysteroid dehydrogenase 2 is essential for steroidogenic activity (2011), Biochemistry, 50, 11015-11024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expressed in Sf9 cells using the baculovirus system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 trilostane
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 mitochondrial inner membrane
-
 Homo sapiens 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 42000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 additional information Homo sapiens using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens P26439
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.145 enzyme interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine. It becomes less stable when incubated with the individual lipids. Dipalmitoylphosphatidylglycerol, alone or in combination with dipalmitoylphosphatidylcholine, leads to a decrease in alpha-helical content without an effect on the beta-sheet conformation. With the exception of the 20 N-terminal amino acids, mixed vesicles protect the enzyme from trypsin digestion. Protein incubated with dipalmitoylphosphatidylcholine is only partially protected Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 additional information using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine Homo sapiens ?
-
 ?
1.1.1.145 additional information enzyme diplays lipid-mediated unfolding. As isoform 3betaHSD2 lacks a receptor, opening the conformation may activate the protein by forming a cavity forms between the alpha-helix and beta-sheet Homo sapiens ?
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Homo sapiens progesterone + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3betaHSD2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.145 37
-
 3betaHSD2 becomes less stable when incubated with the individual lipids dipalmitoylphosphatidylglycerol or dipalmitoylphosphatidylcholine, as indicated by the decrease in thermal denaturation (Tm) from 42 to 37°C. Dipalmitoylphosphatidylglycerol alone or in combination with dipalmitoylphosphatidylcholine, leads to a decrease in alpha-helical content without an effect on the beta-sheet conformation Homo sapiens
1.1.1.145 37
-
 approximately, melting temperature in the presence of zwitterionically charged dipalmitoylphosphatidylcholine vesicles or an equal mixture of zwitterionic vesicles and anionic vesicles Homo sapiens
1.1.1.145 42
-
 melting temperature in the absence of lipid Homo sapiens
1.1.1.145 42
-
 Tm in the absence of any lipid Homo sapiens
1.1.1.145 65
-
 complete denaturation Homo sapiens