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Literature summary extracted from

  • Diaz-Sanchez, A.; Gonzalez-Segura, L.; Rudino-Pinera, E.; Lira-Rocha, A.; Torres-Larios, A.; Munoz-Clares, R.
    Novel NADPH-cysteine covalent adduct found in the active site of an aldehyde dehydrogenase (2011), Biochem. J., 439, 443-452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 expressed in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.8 native and mutant enzyme C286A in complex with NADPH, hanging drop vapor diffusion method, using 85 mM HEPES/NaOH buffer, pH 7.5, 8.5% (v/v) propan-2-ol, 17% (w/v) PEG 4000, and 15% (v/v) glycerol Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 C286A mutant with reduced reactivity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 NAD(P)H reversible inactivation Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NADH + H+
-
 ir
1.2.1.8 betaine aldehyde + NADP+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NADPH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa Q9HTJ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NADH + H+
-
 ir
1.2.1.8 betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NADPH + H+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+ the enzyme can use NADP+ with similar efficiency to NAD+ Pseudomonas aeruginosa
1.2.1.8 NADP+ the enzyme can use NADP+ with similar efficiency to NAD+ Pseudomonas aeruginosa