Literature summary extracted from

Modis, K.; Asimakopoulou, A.; Coletta, C.; Papapetropoulos, A.; Szabo, C.
Oxidative stress suppresses the cellular bioenergetic effect of the 3-mercaptopyruvate sulfurtransferase/hydrogen sulfide pathway (2013), Biochem. Biophys. Res. Commun., 433, 401-407.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.8.1.2	expressed in Escherichia coli BL21(DE3) Codon Plus cells	Mus musculus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.8.1.2	hydrogen peroxide	hydrogen peroxide (0.1-0.5 mM) causes a concentration-dependent decrease in the activity of recombinant enzyme (54% inhibition at 0.1 mM, 87% inhibition at 0.5 mM)	Mus musculus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.8.1.2	mitochondrion	-	Mus musculus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.8.1.2	60000	-	gel filtration	Mus musculus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.8.1.2	3-mercaptopyruvate + cyanide	Mus musculus	-	pyruvate + thiocyanate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.1.2	Mus musculus	-	-	-

Oxidation Stability

EC Number	Oxidation Stability	Organism
2.8.1.2	oxidative stress (0.1-0.5 mM H2O2) inhibits enzyme activity	Mus musculus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.8.1.2	GSTrap FF affinity column chromatography	Mus musculus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.8.1.2	Hepa-1c1c7 cell	-	Mus musculus	-
2.8.1.2	liver	-	Mus musculus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.1.2	3-mercaptopyruvate + cyanide	-	Mus musculus	pyruvate + thiocyanate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.8.1.2	3-MST	-	Mus musculus

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Release 2023.1 (January 2023)