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Literature summary extracted from

  • Bian, L.; Shuai, J.J.; Xiong, F.; Peng, R.H.; Yao, Q.H.; Xiong, A.S.
    Expression, purification, and characterization of a 2,3-dihydroxybiphenyl-1,2-dioxygenase from Bacillus sp. JF8 in Escherichia coli (2012), Biochem. Biophys. Res. Commun., 419, 339-343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 gene BsbphCI, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, the transgenic Escherichia coli strain expressing the BsbphCI gene degrades biphenyl-2,3-diol more quickly than the wild-type strain Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 Ca2+
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 Cr3+
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 Fe2+
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 H2O2 81% inhibition at 10 mM Bacillus sp. (in: Bacteria)
1.13.11.39 KI 43% inhibition at 600 mM Bacillus sp. (in: Bacteria)
1.13.11.39 Li+
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 SDS 46% inhibition at 25 mM Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Cd2+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 Co2+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 Cu2+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 K+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 Mg2+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 Ni2+ activates Bacillus sp. (in: Bacteria)
1.13.11.39 Zn2+ activates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 36000
-
 x * 36000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Bacillus sp. (in: Bacteria)
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Bacillus sp. (in: Bacteria) JF8
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Bacillus sp. (in: Bacteria) Q8GR45 gene BsbphCI
-
1.13.11.39 Bacillus sp. (in: Bacteria) JF8 Q8GR45 gene BsbphCI
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) JF8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 ? x * 36000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-DHBP dioxygenase
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 2,3-dihydroxybiphenyl-1,2-dioxygenase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 50
-
 recombinant enzyme Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.39 20 80 activity range Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.39 60
-
 purified recombinant enzyme, 60 min, 33% activity remaining Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8.5
-
 recombinant enzyme Bacillus sp. (in: Bacteria)

General Information

EC Number General Information Comment Organism
1.13.11.39 physiological function 2,3-dihydroxybiphenyl-1,2-dioxygenase plays an important role in the degradation of polychlorinated biphenyls Bacillus sp. (in: Bacteria)