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Literature summary extracted from
- Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells (2010), Biochem. Biophys. Res. Commun., 402, 750-754.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.35 | expressed in Escherichia coli BL21(DE3) cells | Acinetobacter calcoaceticus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.99.35 | N428C | the catalytic efficiency is increased by 47% compared to the wild type enzyme, the mutant shows increased affinity for pyrroloquinoline quinone and is twice more active toward D-glucose and more selective toward maltose than the wild type | Acinetobacter calcoaceticus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.35 | 43 | - |
D-glucose | wild type enzyme, at pH 7.0 and 37°C | Acinetobacter calcoaceticus |  |
| 1.1.99.35 | 64 | - |
D-glucose | mutant enzyme N428C, at pH 7.0 and 37°C | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.35 | 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.35 | Acinetobacter calcoaceticus | P13650 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.35 | homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.35 | PQQ-GDH | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | soluble PQQ-glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.35 | pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
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Release 2023.1 (January 2023)
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