EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.13 | expression in Escherichia coli | Proteus sp. |
2.8.3.21 | expression in Escherichia coli | Proteus sp. |
4.2.1.149 | - |
Proteus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.149 | acetoacetyl-CoA | 0.1 mM, 25% inhibition | Proteus sp. | |
4.2.1.149 | acetyl-CoA | 0.1 mM, 23% inhibition | Proteus sp. | |
4.2.1.149 | butyryl-CoA | 0.1 mM, 29% inhibition | Proteus sp. | |
4.2.1.149 | CoA | 0.1 mM, 29% inhibition | Proteus sp. | |
4.2.1.149 | decanoyl-CoA | 0.1 mM, 79% inhibition | Proteus sp. | |
4.2.1.149 | gamma-butyrobetainyl-CoA | 0.1 mM, 27% inhibition, competitive inhibitor | Proteus sp. | |
4.2.1.149 | hexanoyl-CoA | 0.1 mM, 43% inhibition | Proteus sp. | |
4.2.1.149 | palmitoyl-CoA | 0.01 mM, 87% inhibition | Proteus sp. | |
4.2.1.149 | succinyl-CoA | 0.1 mM, 22% inhibition | Proteus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.149 | 0.043 | - |
(E)-4-(trimethylammonio)but-2-enoyl-CoA | 37°C, pH 7.5 | Proteus sp. | |
4.2.1.149 | 0.52 | - |
crotonyl-CoA | 37°C, pH 7.5 | Proteus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.13 | 41800 | - |
- |
Proteus sp. |
1.3.8.13 | 166000 | - |
gel filtration | Proteus sp. |
4.2.1.149 | 40000 | - |
3 * 40000, SDS-PAGE | Proteus sp. |
4.2.1.149 | 85700 | - |
gel filtration | Proteus sp. |
6.2.1.48 | 58600 | - |
- |
Proteus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.13 | crotonobetainyl-CoA + reduced electron-transfer flavoprotein | Proteus sp. | - |
gamma-butyrobetainyl-CoA + electron-transfer flavoprotein | - |
ir | |
1.3.8.13 | crotonobetainyl-CoA + reduced electron-transfer flavoprotein | Proteus sp. LE138 | - |
gamma-butyrobetainyl-CoA + electron-transfer flavoprotein | - |
ir | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | Proteus sp. | the enzyme is involved in carnitine metabolism | L-carnitinyl-CoA | - |
r | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | Proteus sp. LE138 | the enzyme is involved in carnitine metabolism | L-carnitinyl-CoA | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.13 | Proteus sp. | Q8GB20 | - |
- |
1.3.8.13 | Proteus sp. LE138 | Q8GB20 | - |
- |
2.8.3.21 | Proteus sp. | Q8GB19 | - |
- |
2.8.3.21 | Proteus sp. LE138 | Q8GB19 | - |
- |
4.2.1.149 | Proteus sp. | Q8GB17 | - |
- |
4.2.1.149 | Proteus sp. LE138 | Q8GB17 | - |
- |
6.2.1.48 | Proteus sp. | Q8GB18 | - |
- |
6.2.1.48 | Proteus sp. LE138 | Q8GB18 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.149 | - |
Proteus sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.8.3.21 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + L-carnitine = (E)-4-(trimethylammonio)but-2-enoate + L-carnitinyl-CoA | sequential bisubstrate mechanism | Proteus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.13 | 6.35 | - |
pH not specified in the publication, temperature not specified in the publication | Proteus sp. |
2.8.3.21 | 556.3 | - |
pH 7.8, 37°C | Proteus sp. |
4.2.1.149 | 6088 | - |
37°C, pH 7.5 | Proteus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.13 | crotonobetainyl-CoA + reduced benzylviologen | - |
Proteus sp. | gamma-butyrobetainyl-CoA + oxidized benzylviologen | - |
ir | |
1.3.8.13 | crotonobetainyl-CoA + reduced benzylviologen | - |
Proteus sp. LE138 | gamma-butyrobetainyl-CoA + oxidized benzylviologen | - |
ir | |
1.3.8.13 | crotonobetainyl-CoA + reduced electron-transfer flavoprotein | - |
Proteus sp. | gamma-butyrobetainyl-CoA + electron-transfer flavoprotein | - |
ir | |
1.3.8.13 | crotonobetainyl-CoA + reduced electron-transfer flavoprotein | - |
Proteus sp. LE138 | gamma-butyrobetainyl-CoA + electron-transfer flavoprotein | - |
ir | |
2.8.3.21 | crotonobetainyl-CoA + L-carnitine | - |
Proteus sp. | crotonobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | crotonobetainyl-CoA + L-carnitine | - |
Proteus sp. LE138 | crotonobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | gamma-butyrobetainyl-CoA + L-carnitine | - |
Proteus sp. | gamma-butyrobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | gamma-butyrobetainyl-CoA + L-carnitine | - |
Proteus sp. LE138 | gamma-butyrobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | additional information | enzyme CaiB acts as betainyl-CoA transferase specific for carnitine, crotonobetaine, gamma-butyrobetaine and its CoA derivatives. Transferase reaction proceeds, following a sequential bisubstrate mechanism. No substrates: CoA, acetoacetyl-CoA, acetyl-CoA, crotonyl-CoA, hydroxybutyryl-CoA, palmitoyl-CoA, succinyl-CoA | Proteus sp. | ? | - |
? | |
2.8.3.21 | additional information | enzyme CaiB acts as betainyl-CoA transferase specific for carnitine, crotonobetaine, gamma-butyrobetaine and its CoA derivatives. Transferase reaction proceeds, following a sequential bisubstrate mechanism. No substrates: CoA, acetoacetyl-CoA, acetyl-CoA, crotonyl-CoA, hydroxybutyryl-CoA, palmitoyl-CoA, succinyl-CoA | Proteus sp. LE138 | ? | - |
? | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | the enzyme is involved in carnitine metabolism | Proteus sp. | L-carnitinyl-CoA | - |
r | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | (E)-4-(trimethylammonio)but-2-enoyl-CoA i.e. crotonobetainyl-CoA | Proteus sp. | L-carnitinyl-CoA | L-carnitinyl-CoA i.e. (3R)-3-hydroxy-4-(trimethylammonio)butanoyl-CoA | r | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | the enzyme is involved in carnitine metabolism | Proteus sp. LE138 | L-carnitinyl-CoA | - |
r | |
4.2.1.149 | (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O | (E)-4-(trimethylammonio)but-2-enoyl-CoA i.e. crotonobetainyl-CoA | Proteus sp. LE138 | L-carnitinyl-CoA | L-carnitinyl-CoA i.e. (3R)-3-hydroxy-4-(trimethylammonio)butanoyl-CoA | r | |
4.2.1.149 | crotonyl-CoA + H2O | crotonyl-CoA is hydrated with low efficiency than (E)-4-(trimethylammonio)but-2-enoyl-CoA | Proteus sp. | ? | - |
? | |
4.2.1.149 | crotonyl-CoA + H2O | crotonyl-CoA is hydrated with low efficiency than (E)-4-(trimethylammonio)but-2-enoyl-CoA | Proteus sp. LE138 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.13 | tetramer | 4 * 41800, SDS-PAGE | Proteus sp. |
4.2.1.149 | homotrimer | 3 * 40000, SDS-PAGE | Proteus sp. |
6.2.1.48 | ? | x * 58600, calculated | Proteus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.149 | CaiD | - |
Proteus sp. |
4.2.1.149 | L-carnityl-CoA dehydratase | - |
Proteus sp. |
6.2.1.48 | caiC | - |
Proteus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.149 | 0.068 | - |
gamma-butyrobetainyl-CoA | 37°C, pH 7.5 | Proteus sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.149 | physiological function | the enzyme is involved in carnitine metabolism | Proteus sp. |
6.2.1.48 | physiological function | enzyme is part of the caiTABCDEF operon. The caiA gene product is a crotonobetainyl-CoA reductase catalysing the irreversible reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. CaiB acts as betainyl-CoA transferase specific for carnitine, crotonobetaine, gamma-butyrobetaine and its CoA derivatives. CaiD is a crotonobetainyl-CoA hydratase, and CaiC is suggested to be a betainyl-CoA ligase | Proteus sp. |