EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.7 | expressed in Escherichia coli BL21 (DE3)-RIL cells | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.7 | E16Q/C205S | the mutation decreases the turnover number by about 15fold | Saccharomyces cerevisiae |
1.5.1.7 | K13M/C205S | the mutation decreases the turnover number by about 15fold | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.7 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Saccharomyces cerevisiae | - |
L-lysine + 2-oxoglutarate + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.7 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.7 | Ni2+-NTA column chromatography | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.7 | 4°C, His-tagged mutant enzymes in 100 mM HEPES, 300 mM KCl and 300 mM imidazole at pH 8.0, several months, the mutant enzymes maintain stability and remain active | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.7 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-lysine + 2-oxoglutarate + NADH | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.7 | N6-(glutaryl-2)-L-lysine: NAD+ oxidoreductase | - |
Saccharomyces cerevisiae |
1.5.1.7 | SDH | - |
Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.7 | NAD+ | - |
Saccharomyces cerevisiae |