Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Feng, J.; Kweon, O.; Xu, H.; Cerniglia, C.E.; Chen, H.
    Probing the NADH- and Methyl Red-binding site of a FMN-dependent azoreductase (AzoA) from Enterococcus faecalis (2012), Arch. Biochem. Biophys., 520, 99-107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6
-
 Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.1.6 modeling of structure. Binding mode shows that the benzoic acid moiety of substrate methyl red and the nicotinamide ring of NADH are not parallel to the flavin isoalloxazine ring, but lay against it at angles of about 45 and 35 degrees, respectively. The adenine ribose moiety of NADH is surrounded by loop l2 on chain B and alpha3 on chain A in a typical Rossmann fold Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.6 A123F 36% of wild-type activity Enterococcus faecalis
1.7.1.6 D184G complete loss of activity Enterococcus faecalis
1.7.1.6 E16G 46% of wild-type activity Enterococcus faecalis
1.7.1.6 F127G complete loss of activity Enterococcus faecalis
1.7.1.6 L59G 10% of wild-type activity Enterococcus faecalis
1.7.1.6 N106A 3% of wild-type activity Enterococcus faecalis
1.7.1.6 N121A 170% of wild-type activity Enterococcus faecalis
1.7.1.6 R18G 9% of wild-type activity Enterococcus faecalis
1.7.1.6 R21G 382% of wild-type activity Enterococcus faecalis
1.7.1.6 R66A 47% of wild-type activity Enterococcus faecalis
1.7.1.6 V122Y 14% of wild-type activity Enterococcus faecalis
1.7.1.6 W62A 35% of wild-type activity Enterococcus faecalis
1.7.1.6 Y129G complete loss of activity Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.00053
-
 Methyl red mutant R66A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.003
-
 NADH mutant W62A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0082
-
 Methyl red mutant N121A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0112
-
 Methyl red mutant N106A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0126
-
 Methyl red mutant R21G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0167
-
 Methyl red mutant R18G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.024
-
 Methyl red wild-type, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0288
-
 Methyl red mutant L59G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0335
-
 Methyl red mutant W62A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.0485
-
 Methyl red mutant A123F, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.109
-
 Methyl red mutant V122Y, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.143
-
 NADH wild-type, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.25
-
 NADH mutant E16G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.292
-
 NADH mutant N121A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.38
-
 NADH mutant R21G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.405
-
 NADH mutant V122Y, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.411
-
 NADH mutant A123F, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.581
-
 NADH mutant R66A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.624
-
 NADH mutant R18G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 0.793
-
 NADH mutant N106A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 2.298
-
 NADH mutant L59G, pH 7.0, 22°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Enterococcus faecalis Q831B2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 methyl red + NADH + H+
-
 Enterococcus faecalis ? + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoA
-
 Enterococcus faecalis
1.7.1.6 AzoR
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.6 1.2
-
 Methyl red mutant N106A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 3.17
-
 Methyl red mutant R18G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 6.61
-
 Methyl red mutant L59G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 17.5
-
 Methyl red mutant V122Y, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 20.8
-
 Methyl red mutant E16G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 21.1
-
 Methyl red mutant W62A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 59.1
-
 Methyl red wild-type, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 77.3
-
 Methyl red mutant R66A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 111
-
 Methyl red mutant N121A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 140
-
 Methyl red mutant R21G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 245
-
 Methyl red mutant A123F, pH 7.0, 22°C Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN
-
 Enterococcus faecalis
1.7.1.6 NADH
-
 Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.6 1.5
-
 NADH mutant N106A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 2.9
-
 NADH mutant L59G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 5
-
 NADH mutant R18G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 44
-
 NADH mutant V122Y, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 83
-
 NADH mutant E16G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 110
-
 Methyl red mutant N106A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 111
-
 Methyl red mutant R21G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 130
-
 NADH mutant R66A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 160
-
 Methyl red mutant V122Y, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 190
-
 Methyl red mutant R18G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 230
-
 Methyl red mutant L59G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 370
-
 NADH mutant R21G, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 380
-
 NADH mutant N121A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 410
-
 NADH wild-type, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 600
-
 NADH mutant A123F, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 630
-
 Methyl red mutant W62A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 2460
-
 Methyl red wild-type, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 5050
-
 Methyl red mutant A123F, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 7030
-
 NADH mutant W62A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 13540
-
 Methyl red mutant N121A, pH 7.0, 22°C Enterococcus faecalis
1.7.1.6 14580
-
 Methyl red mutant R66A, pH 7.0, 22°C Enterococcus faecalis