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Literature summary extracted from

  • Sygmund, C.; Gutmann, A.; Krondorfer, I.; Kujawa, M.; Glieder, A.; Pscheidt, B.; Haltrich, D.; Peterbauer, C.; Kittl, R.
    Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris (2012), Appl. Microbiol. Biotechnol., 94, 695-704.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Pichia pastoris strain CBS 7435 Leucoagaricus meleagris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.13
-
 ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.16
-
 ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.49
-
 L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.54
-
 L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.55
-
 2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.62
-
 2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.69
-
 D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.82
-
 D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.05
-
 D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.07
-
 D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.38
-
 1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 1.82
-
 1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
1.1.99.29 1.92
-
 D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.93
-
 D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 128
-
 lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 134
-
 lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 61967
-
 x * 61967, calculated from amino acid sequence Leucoagaricus meleagris
1.1.99.29 64000
-
 x * 64000, deglycosylated protein, SDS-PAGE Leucoagaricus meleagris
1.1.99.29 93000
-
 x * 93000, recombinant enzyme, SDS-PAGE Leucoagaricus meleagris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.29 D-galactose + ferrocenium ion Leucoagaricus meleagris
-
 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone Leucoagaricus meleagris
-
 ?
-
 ?
1.1.99.29 D-glucose + 2-chloro-1,4-benzoquinone Leucoagaricus meleagris
-
 ?
-
 ?
1.1.99.29 D-glucose + ferrocenium ion Leucoagaricus meleagris
-
 3-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-xylose + ferrocenium ion Leucoagaricus meleagris
-
 ? + ferrocene
-
 ?
1.1.99.29 L-arabinose + ferrocenium ion Leucoagaricus meleagris
-
 ? + ferrocene
-
 ?
1.1.99.29 lactose + ferrocenium ion Leucoagaricus meleagris
-
 ? + ferrocene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein the level of glycosylation of recombinant enzyme is approximately 30% Leucoagaricus meleagris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 DEAE Sepharose column chromatography and phenyl Sepharose column chromatography Leucoagaricus meleagris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.29 11.5
-
 culture supernatant, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 21.9
-
 after 1.9fold purification, at pH 8.5 and 30°C Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 D-galactose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
 Leucoagaricus meleagris ?
-
 ?
1.1.99.29 D-glucose + 2-chloro-1,4-benzoquinone
-
 Leucoagaricus meleagris ?
-
 ?
1.1.99.29 D-glucose + ferrocenium ion
-
 Leucoagaricus meleagris 3-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-xylose + ferrocenium ion
-
 Leucoagaricus meleagris ? + ferrocene
-
 ?
1.1.99.29 L-arabinose + ferrocenium ion
-
 Leucoagaricus meleagris ? + ferrocene
-
 ?
1.1.99.29 lactose + ferrocenium ion
-
 Leucoagaricus meleagris ? + ferrocene
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 ? x * 61967, calculated from amino acid sequence Leucoagaricus meleagris
1.1.99.29 ? x * 64000, deglycosylated protein, SDS-PAGE Leucoagaricus meleagris
1.1.99.29 ? x * 93000, recombinant enzyme, SDS-PAGE Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH1
-
 Leucoagaricus meleagris
1.1.99.29 pyranose dehydrogenase
-
 Leucoagaricus meleagris
1.1.99.29 pyranose/acceptor oxidoreductase
-
 Leucoagaricus meleagris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 12.9
-
 2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 15.1
-
 2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 34.7
-
 L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 37.2
-
 L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 37.8
-
 D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 39.6
-
 lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 41
-
 lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 43.4
-
 D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 45.9
-
 D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 47.3
-
 D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 48.5
-
 D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 62.4
-
 D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 65.4
-
 1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 76
-
 1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
1.1.99.29 104
-
 ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 130
-
 ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 flavin
-
 Leucoagaricus meleagris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.29 0.29
-
 lactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.32
-
 lactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 20.9
-
 2-chloro-1,4-benzoquinone recombinant enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 22.9
-
 D-xylose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 25.5
-
 2-chloro-1,4-benzoquinone native enzyme, at pH 5.0 and 30°C Leucoagaricus meleagris
1.1.99.29 32.5
-
 D-xylose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 44.2
-
 D-galactose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 46.2
-
 D-galactose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 54.4
-
 1,4-benzoquinone recombinant enzyme, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 54.8
-
 D-glucose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 57.5
-
 1,4-benzoquinone native enzyme, at pH 3.0 and 30°C Leucoagaricus meleagris
1.1.99.29 57.5
-
 D-glucose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 62.1
-
 L-arabinose using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 70.8
-
 L-arabinose using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 802
-
 ferrocenium ion native enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 812
-
 ferrocenium ion recombinant enzyme, at pH 8.5 and 30°C Leucoagaricus meleagris