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Literature summary extracted from

  • Kawai, S.; Goda-Tsutsumi, M.; Yakushi, T.; Kano, K.; Matsushita, K.
    Heterologous overexpression and characterization of a flavoprotein-cytochrome c complex fructose dehydrogenase of Gluconobacter japonicus NBRC3260 (2013), Appl. Environ. Microbiol., 79, 1654-1660.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.14 membrane
-
 Gluconobacter japonicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.14 16000
-
 1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence Gluconobacter japonicus
1.1.5.14 18000
-
 1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE Gluconobacter japonicus
1.1.5.14 49000
-
 1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence Gluconobacter japonicus
1.1.5.14 51000
-
 1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE Gluconobacter japonicus
1.1.5.14 60000
-
 1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence Gluconobacter japonicus
1.1.5.14 68000
-
 1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE Gluconobacter japonicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.14 Gluconobacter japonicus
-
 formerly Gluconobacter industrius strain IFO3260
-
1.1.5.14 Gluconobacter japonicus NBRC3260
-
 formerly Gluconobacter industrius strain IFO3260
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.14 DEAE-Sepharose column chromatography Gluconobacter japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.14 D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone
-
 Gluconobacter japonicus 5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol
-
 ?
1.1.5.14 D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone
-
 Gluconobacter japonicus NBRC3260 5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol
-
 ?
1.1.5.14 D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 Gluconobacter japonicus 5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
 ?
1.1.5.14 D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 Gluconobacter japonicus NBRC3260 5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
 ?
1.1.5.14 additional information cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction Gluconobacter japonicus ?
-
 ?
1.1.5.14 additional information cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction Gluconobacter japonicus NBRC3260 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.14 heterotrimer 1 * 60000 + 1 * 49000 + 1 * 16000, calculated from amino acid sequence Gluconobacter japonicus
1.1.5.14 heterotrimer 1 * 68000 + 1 * 51000 + 1 * 18000, SDS-PAGE Gluconobacter japonicus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.14 FDH
-
 Gluconobacter japonicus
1.1.5.14 FdhL large subunit Gluconobacter japonicus
1.1.5.14 FdhS small subunit Gluconobacter japonicus
1.1.5.14 fdhSCL gene name of the flavoprotein-cytochrome c complex fructose dehydrogenase Gluconobacter japonicus
1.1.5.14 fructose dehydrogenase
-
 Gluconobacter japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.14 4
-
-
 Gluconobacter japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.14 cytochrome c the cytochrome c subunit is responsible not only for membrane anchoring but also for ubiquinone reduction Gluconobacter japonicus
1.1.5.14 heme the enzyme has three heme C moieties Gluconobacter japonicus