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Literature summary extracted from

  • Preety, S.C.; Hooda, V.
    Immobilization and kinetics of catalase on calcium carbonate nanoparticles attached epoxy support (2014), Appl. Biochem. Biotechnol., 172, 115-130.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.6 additional information immobilization and kinetics of catalase on calcium carbonate nanoparticles attached epoxy support, synthesized by miniemulsion technique, overview. A decrease in Vmax value from 1.50 to 0.42 mM/mg protein is observed after immobilization. Thermal and storage stabilities of catalase improved immensely after immobilization. The immobilized enzyme retains three times than the activity of free enzyme when kept at 75°C for 1 h and the half-life of enzyme increases five times when stored in 0.01 M phosphate, pH 7.0, at 5°C. The enzyme can be reused 30times without any significant loss of its initial activity Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 27.73
-
H2O2 pH 7.0, 25°C, free enzyme Aspergillus niger
1.11.1.6 31.42
-
H2O2 pH 7.0, 25°C, immobilized enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 2 H2O2 Aspergillus niger
-
O2 + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 commercial preparation
-
Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 2 H2O2
-
Aspergillus niger O2 + 2 H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 35
-
free enzyme and immobilized enzyme on nanoparticles Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.6 15 75 activity range, free enzyme and immobilized enzyme on nanoparticles Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7
-
free enzyme and immobilized enzyme on nanoparticles Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 5 9 activity range, free enzyme and immobilized enzyme on nanoparticles Aspergillus niger