EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.1.2 | thioredoxin | rat-reduced thioredoxin activates the enzyme 2.3fold after treatment with dithiothreitol, but dithiothreitol does not increase enzyme activity. The Escherichia coli thioredoxin-thioredoxin reductase-NADPH system more effectively increases enzyme activity to 4.5fold that of the control than the rat thioredoxin-thioredoxin reductase-NADPH system, which increases enzyme activity to 3fold that of the control | Mus musculus | |
2.8.1.2 | thioredoxin | rat-reduced thioredoxin activates the enzyme 2.3fold after treatment with dithiothreitol, but dithiothreitol does not increase enzyme activity. The Escherichia coli thioredoxin-thioredoxin reductase-NADPH system more effectively increases enzyme activity to 4.5fold that of the control than the rat thioredoxin-thioredoxin reductase-NADPH system, which increases enzyme activity to 3fold that of the control | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.8.1.2 | C154S | active site mutant | Mus musculus |
2.8.1.2 | C154S | active site mutant | Rattus norvegicus |
2.8.1.2 | C263S | active site mutant | Mus musculus |
2.8.1.2 | C263S | active site mutant | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.1.2 | iodoacetate | - |
Mus musculus | |
2.8.1.2 | iodoacetate | - |
Rattus norvegicus | |
2.8.1.2 | tetrathionate | reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system | Mus musculus | |
2.8.1.2 | tetrathionate | reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.1.2 | cytosol | - |
Mus musculus | 5829 | - |
2.8.1.2 | cytosol | - |
Rattus norvegicus | 5829 | - |
2.8.1.2 | mitochondrion | - |
Mus musculus | 5739 | - |
2.8.1.2 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.1.2 | 32800 | - |
2 * 32800, calculated from amino acid sequence | Mus musculus |
2.8.1.2 | 32800 | - |
2 * 32800, calculated from amino acid sequence | Rattus norvegicus |
2.8.1.2 | 33000 | - |
2 * 33000, SDS-PAGE | Mus musculus |
2.8.1.2 | 33000 | - |
2 * 33000, SDS-PAGE | Rattus norvegicus |
2.8.1.2 | 66600 | - |
gel filtration | Mus musculus |
2.8.1.2 | 66600 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.2 | 3-mercaptopyruvate + cyanide | Mus musculus | - |
pyruvate + thiocyanate | - |
? | |
2.8.1.2 | 3-mercaptopyruvate + cyanide | Rattus norvegicus | - |
pyruvate + thiocyanate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.1.2 | Mus musculus | - |
- |
- |
2.8.1.2 | Rattus norvegicus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.1.2 | liver | - |
Mus musculus | - |
2.8.1.2 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.2 | 3-mercaptopyruvate + cyanide | - |
Mus musculus | pyruvate + thiocyanate | - |
? | |
2.8.1.2 | 3-mercaptopyruvate + cyanide | - |
Rattus norvegicus | pyruvate + thiocyanate | - |
? | |
2.8.1.2 | additional information | in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate | Mus musculus | ? | - |
? | |
2.8.1.2 | additional information | in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.1.2 | homodimer | 2 * 33000, SDS-PAGE | Mus musculus |
2.8.1.2 | homodimer | 2 * 33000, SDS-PAGE | Rattus norvegicus |
2.8.1.2 | homodimer | 2 * 32800, calculated from amino acid sequence | Mus musculus |
2.8.1.2 | homodimer | 2 * 32800, calculated from amino acid sequence | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.1.2 | mercaptopyruvate sulfurtransferase | - |
Mus musculus |
2.8.1.2 | mercaptopyruvate sulfurtransferase | - |
Rattus norvegicus |
2.8.1.2 | MST | - |
Mus musculus |
2.8.1.2 | MST | - |
Rattus norvegicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.8.1.2 | malfunction | mercaptolactate-cysteine disulfiduria is caused by enzyme defect with or without mental retardation | Mus musculus |
2.8.1.2 | physiological function | the enzyme serves not only as an enzyme in cysteine catabolism, but also as an antioxidant protein | Mus musculus |
2.8.1.2 | physiological function | the enzyme serves not only as an enzyme in cysteine catabolism, but also as an antioxidant protein | Rattus norvegicus |