Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ngo, H.P.; Hong, S.H.; Oh, D.K.; Kang, L.W.
    Expression, crystallization and preliminary X-ray crystallographic analysis of aldehyde dehydrogenase (ALDH) from Bacillus cereus (2013), Acta Crystallogr. Sect. F, 69, 528-531.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli ER2566 cells Bacillus cereus
1.2.1.5 expressed in Escherichia coli ER2566 cells Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.3 hanging drop vapor diffusion method, using 0.15 M DL-malic acid pH 7.0, 18%(w/v) PEG 3350 Bacillus cereus
1.2.1.5 hanging drop vapor diffusion method, using 0.15 M DL-malic acid pH 7.0, 18% (w/v) PEG 3350 Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 55600
-
 4 * 55600, recombinant enzyme plus 13 additional residues from the pRSF Duct-1 vector, SDS-PAGE Bacillus cereus
1.2.1.5 54000
-
 4 * 54000, SDS-PAGE Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Bacillus cereus
-
-
-
1.2.1.3 Bacillus cereus ATCC 10876
-
-
-
1.2.1.5 Bacillus cereus A0A150BLG9
-
-
1.2.1.5 Bacillus cereus ATCC 10876 A0A150BLG9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 immobilized metal-ion affinity column chromatography Bacillus cereus
1.2.1.5 immobilized metal ion affinity column chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Bacillus cereus acetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 acetate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homotetramer 4 * 55600, recombinant enzyme plus 13 additional residues from the pRSF Duct-1 vector, SDS-PAGE Bacillus cereus
1.2.1.5 homotetramer 4 * 54000, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH
-
 Bacillus cereus
1.2.1.5 ALDH
-
 Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Bacillus cereus
1.2.1.5 NAD+
-
 Bacillus cereus
1.2.1.5 NADP+
-
 Bacillus cereus