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Literature summary extracted from

  • Tourigny, D.S.; Elliott, P.R.; Edgell, L.J.; Hudson, G.M.; Moody, P.C.
    Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni (2011), Acta Crystallogr. Sect. F, 67, 72-75.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.59 expressed in Escherichia coli Rosetta DE3 cells Campylobacter jejuni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.59 wild type and mutant enzyme C150S, sitting drop vapor diffusion method, using 25% (w/v) PEG 1K, 175 mM sodium/potassium tartrate and 100 mM sodium acetate pH 4.5 Campylobacter jejuni

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.59 C150S the mutant is unable to turn over D-glyceraldehyde 3-phosphate in the presence of either NAD+ or NADP+ Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+ Campylobacter jejuni
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+ Campylobacter jejuni NCTC 11168
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NADP+ Campylobacter jejuni
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NADP+ Campylobacter jejuni NCTC 11168
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.59 Campylobacter jejuni
-
-
-
1.2.1.59 Campylobacter jejuni NCTC 11168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.59 Hi-Trap nickel Sepharose column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Campylobacter jejuni 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Campylobacter jejuni NCTC 11168 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Campylobacter jejuni 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Campylobacter jejuni NCTC 11168 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.59 cgap
-
 Campylobacter jejuni
1.2.1.59 GAPDH
-
 Campylobacter jejuni

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.59 NAD+ the enzyme can utilize either NADP+ or NAD+ as coenzymes Campylobacter jejuni
1.2.1.59 NADP+ the enzyme can utilize either NADP+ or NAD+ as coenzymes Campylobacter jejuni