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Literature summary extracted from
- Structure of recombinant Leishmania donovani pteridine reductase reveals a disordered active site (2011), Acta Crystallogr. Sect. F, 67, 33-37.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.33 | expressed in Escherichia coli BL21 (DE3) GOLD cells | Leishmania donovani |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.5.1.33 | sitting drop vapor diffusion method, using 0.1 M MES pH 6.5, 10%(v/v) dioxane and 1.6 M ammonium sulfate | Leishmania donovani |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.33 | methotrexate | - |
Leishmania donovani |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.33 | biopterin + 2 NADPH + 2 H+ | Leishmania donovani | - |
5,6,7,8-tetrahydrobiopterin + 2 NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.33 | Leishmania donovani | Q6QDB5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.33 | HisTrap nickel-chelating column chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.33 | biopterin + 2 NADPH + 2 H+ | - |
Leishmania donovani | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.33 | homotetramer | x-ray crystallography | Leishmania donovani |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.1.33 | NADPH-dependent short-chain dehydrogenase/reductase pteridine reductase | - |
Leishmania donovani |
| 1.5.1.33 | PTR1 | - |
Leishmania donovani |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.33 | NADPH | - |
Leishmania donovani | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.33 | 0.000039 | - |
methotrexate | pH and temperature not specified in the publication | Leishmania donovani | |
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Release 2023.1 (January 2023)
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