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Literature summary extracted from

  • Yamamura, A.; Maruoka, S.; Ohtsuka, J.; Miyakawa, T.; Nagata, K.; Kataoka, M.; Kitamura, N.; Shimizu, S.; Tanokura, M.
    Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708 (2009), Acta Crystallogr. Sect. F, 65, 1145-1148.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.358 expressed in Escherichia coli Rosetta (DE3) cells Candida parapsilosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.358 sitting drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.1 and 23% (w/v) PEG 3350 Candida parapsilosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.358 40000
-
 x * 40000, SDS-PAGE Candida parapsilosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.358 2-dehydropantolactone + NADPH + H+ Candida parapsilosis
-
 (R)-pantolactone + NADP+
-
 ?
1.1.1.358 2-dehydropantolactone + NADPH + H+ Candida parapsilosis IFO 0708
-
 (R)-pantolactone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.358 Candida parapsilosis Q76L36
-
-
1.1.1.358 Candida parapsilosis IFO 0708 Q76L36
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.358 Ni Sepharose column chromatography and Superdex 75 gel filtration Candida parapsilosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.358 2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis (R)-pantolactone + NADP+
-
 ?
1.1.1.358 2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis IFO 0708 (R)-pantolactone + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.358 ? x * 40000, SDS-PAGE Candida parapsilosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.358 conjugated polyketone reductase C2
-
 Candida parapsilosis
1.1.1.358 CPR-C2
-
 Candida parapsilosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.358 NADPH
-
 Candida parapsilosis