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Literature summary extracted from

  • Yuzugullu, Y.; Trinh, C.H.; Fairhurst, L.; Ogel, Z.B.; McPherson, M.J.; Pearson, A.R.
    Investigating the active centre of the Scytalidium thermophilum catalase (2013), Acta Crystallogr. Sect. D, 69, 369-375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) Mycothermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.6 native and recombinant wild-type enzyme and enzyme mutants H82N and V123F, sitting drop vapour diffusion method, mixing of 9.4 mg/ml protein with reservoir solution containing 18% w/v PEG 2000, 0.1 M barium chloride, 0.1 M bis-tris pH 6.8 for the wild-type enzyme, and 6-16% w/v PEG 400, 0.2 M potassium chloride, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 5.0-5.6 for the mutant H82N or PEG 4000 instead of PEG 400 for the mutant V123F, X-ray diffraction structure determination and analysis at 2.7 A, 1.4 A, 1.5 A, and 1.9 A resolution, respectively Mycothermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.6 H82N site-directed mutagenesis, the mutation results in conversion of the native d-type heme to a b-type heme Mycothermus thermophilus
1.11.1.6 V123F site-directed mutagenesis Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 79000
-
 4 * 79000, wild-type and mutant enzymes, SDS-PAGE Mycothermus thermophilus
1.11.1.6 320000
-
-
 Mycothermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 2 H2O2 Mycothermus thermophilus
-
 O2 + 2 H2O
-
 ?
1.11.1.6 2 H2O2 Mycothermus thermophilus ATCC 16454
-
 O2 + 2 H2O
-
 ?
1.11.1.6 additional information Mycothermus thermophilus the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds ?
-
 ?
1.11.1.6 additional information Mycothermus thermophilus ATCC 16454 the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Mycothermus thermophilus
-
 gene catpo
-
1.11.1.6 Mycothermus thermophilus ATCC 16454
-
 gene catpo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) to homogeneity by anion exchange chromatography and gel filtration Mycothermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.6 2 H2O2 = O2 + 2 H2O reaction mechanism, overview Mycothermus thermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 4
-
 mutant H82N, pH 7.0, 60°C Mycothermus thermophilus
1.11.1.6 65
-
 mutant V123F, pH 7.0, 60°C Mycothermus thermophilus
1.11.1.6 8870
-
 native wild-type enzyme, pH 7.0, 60°C Mycothermus thermophilus
1.11.1.6 18710
-
 recombinant wild-type enzyme, pH 7.0, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 2 H2O2
-
 Mycothermus thermophilus O2 + 2 H2O
-
 ?
1.11.1.6 2 H2O2 via Compound I and Compound II intermediates Mycothermus thermophilus O2 + 2 H2O
-
 ?
1.11.1.6 2 H2O2
-
 Mycothermus thermophilus ATCC 16454 O2 + 2 H2O
-
 ?
1.11.1.6 2 H2O2 via Compound I and Compound II intermediates Mycothermus thermophilus ATCC 16454 O2 + 2 H2O
-
 ?
1.11.1.6 additional information the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds Mycothermus thermophilus ?
-
 ?
1.11.1.6 additional information the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds Mycothermus thermophilus ATCC 16454 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 homotetramer 4 * 79000, wild-type and mutant enzymes, SDS-PAGE Mycothermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 CATPO
-
 Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 60
-
 assay at Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7
-
 assay at Mycothermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 additional information the heme component is cis-hydroxychlorin gamma-spirolactone Mycothermus thermophilus

General Information

EC Number General Information Comment Organism
1.11.1.6 additional information the heme-binding pocket contains two highly conserved water molecules on the distal side Mycothermus thermophilus