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Literature summary extracted from

  • Furlong, S.E.; Valvano, M.A.
    Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase (2012), Protein Sci., 21, 1366-1375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.33 expressed in Escherichia coli MV501 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.33 D217A the mutation results in reduced enzymatic activity Escherichia coli
2.7.8.33 D217E the mutation results in a slightly more active enzyme Escherichia coli
2.7.8.33 D217K inactive Escherichia coli
2.7.8.33 D217N the mutation results in a more active enzyme and leads to more than 2fold increase in Vmax without significant change in the Km for the UDP-N-acetyl-D-glucosamine Escherichia coli
2.7.8.33 D217S the mutation results in a more active enzyme Escherichia coli
2.7.8.33 F214A the mutation results in severely reduced enzymatic activity Escherichia coli
2.7.8.33 G216A inactive Escherichia coli
2.7.8.33 M215A the mutation results in a slightly more active enzyme Escherichia coli
2.7.8.33 V213A the mutation results in severely reduced enzymatic activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.33 tunicamycin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.33 0.00007
-
 UDP-N-acetyl-D-glucosamine mutant enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37°C Escherichia coli
2.7.8.33 0.00008
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.33 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.33 Mg2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.33 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.33 Co2+ affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.33 UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate
-
 Escherichia coli UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.33 PNPT
-
 Escherichia coli
2.7.8.33 polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase
-
 Escherichia coli
2.7.8.33 WecA
-
 Escherichia coli