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Literature summary extracted from

  • de la Roche, M.; Tessier, S.N.; Storey, K.B.
    Structural and functional properties of glycerol-3-phosphate dehydrogenase from a mammalian hibernator (2012), Protein J., 31, 109-119.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.001
-
 NADH pH 7.5, 22°C, liver isozyme Cynomys ludovicianus
1.1.1.8 0.001
-
 NADH pH 7.5, 5°C, liver isozyme Cynomys ludovicianus
1.1.1.8 0.001
-
 NADH pH 7.5, 5°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.003
-
 NADH pH 7.5, 22°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.045
-
 NAD+ pH 8.5, 22°C, liver isozyme Cynomys ludovicianus
1.1.1.8 0.09
-
 NAD+ pH 8.5, 5°C, liver isozyme Cynomys ludovicianus
1.1.1.8 0.143
-
 NAD+ pH 8.5, 22°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.154
-
 glycerone phosphate pH 7.5, 5°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.155
-
 NAD+ pH 8.5, 5°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.259
-
 glycerone phosphate pH 7.5, 5°C, liver isozyme Cynomys ludovicianus
1.1.1.8 0.279
-
 glycerone phosphate pH 7.5, 22°C, muscle isozyme Cynomys ludovicianus
1.1.1.8 0.352
-
 glycerone phosphate pH 7.5, 22°C, liver isozyme Cynomys ludovicianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 40000
-
 2 * 40000, SDS-PAGE Cynomys ludovicianus
1.1.1.8 77000
-
 gel filtration Cynomys ludovicianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Cynomys ludovicianus
-
 glycerone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Cynomys ludovicianus
-
 from Southeastern Wyoming
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 native enzyme 50fold and 176fold from liver and muscle, respectively, by anion exchange chromatography, gel filtration, and affinity chromatography Cynomys ludovicianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 liver liver isozyme Cynomys ludovicianus
-
1.1.1.8 skeletal muscle hibernator muscle, muscle isozyme Cynomys ludovicianus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 additional information
-
 activities with the different isozymes at different temperatures, overview Cynomys ludovicianus
1.1.1.8 257
-
 purified enzyme, from liver, pH 8.5, 25°C Cynomys ludovicianus
1.1.1.8 282
-
 purified enzyme, from muscle, pH 8.5, 25°C Cynomys ludovicianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Cynomys ludovicianus glycerone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 40000, SDS-PAGE Cynomys ludovicianus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.8 G3PDH
-
 Cynomys ludovicianus
1.1.1.8 glycerol-3-phosphate dehydrogenase
-
 Cynomys ludovicianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.8 25
-
 assay at Cynomys ludovicianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 7.5
-
 assay at, reduction reaction Cynomys ludovicianus
1.1.1.8 8.5
-
 assay at, oxidation reaction Cynomys ludovicianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Cynomys ludovicianus
1.1.1.8 NADH
-
 Cynomys ludovicianus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.8 Cynomys ludovicianus isoelectric focusing
-
 6.4

General Information

EC Number General Information Comment Organism
1.1.1.8 physiological function G3PDH is an important locus in fuel catabolism in hibernating species Cynomys ludovicianus